Effect of hyperthermia on Id-1,HSF1 expression in Cal27 cell line

doi:10.19438/j.cjoms.2018.02.009

Abstract

Abstract: PURPOSE: To investigate the influence of hyperthermia on expression of Id-1, HSF1 in Cal27 cell and the effect on Cal27 cell proliferation and migration. METHODS: Cal27 cell lines were hyperthermia-treated (43℃ for 1 h); 4 h later, real-time quantitative transcriptase-polymerase chain reaction was used to detect the expressions of Id-1, HSF1. Cell proliferation and migration were observed by CCK-8 and wound healing. SPSS 19.0 software package was used to analyze the data. RESULTS: The mRNA level of Id-1 was decreased and the mRNA level of HSF1 was increased after hyperthermia. The proliferation of Cal27 cell descended at 2 h post-hyperthermia. Migration of Cal27 cell descended after hyperthermia treatment. The difference was statistically significant (P<0.05). CONCLUSIONS: Hyperthermia can inhibit Cal27 cell proliferation and migration ability, reduce the mRNA level of Id-1 and increase the mRNA level of HSF1.

Key words: Hyperthermia, Id-1, HSF1, Tongue squamous cell carcinoma, Cal27

CLC Number:

R739.8

SUN Jun-wei, LUO Dan, LIU Hao, WANG Sheng-zhi. Effect of hyperthermia on Id-1,HSF1 expression in Cal27 cell line[J]. China Journal of Oral and Maxillofacial Surgery, 2018, 16(2): 144-148.

References

[1] Gao S, Zheng M, Ren X, et al.Local hyperthermia in head and neck cancer: mechanism, application and advance[J]. Oncotarget, 2016, 7(35): 57367-57378.
[2] Benezra R, Davis RL, Lockshon D, et al.The protein Id: a negative regulator of helix-loop-helix DNA binding proteins[J]. Cell, 1990, 61(1): 49-59.
[3] Zhao J, Wang S, Liu N, et al.Correlation between the expression of Id-1 and hyperthermia-associated molecules in oral squamous cell carcinoma[J]. J Clin Pathol, 2013, 66(9): 758-763.
[4] Wazir U, Jiang WG, Sharma AK, et al.The mRNA expression of inhibitors of DNA binding-1 and -2 is associated with advanced tumour stage and adverse clinical outcome in human breast cancer[J]. Anticancer Res, 2013, 33(5): 2179-2183.
[5] Ponz-Sarvisé M, Castanoñ E, Panizo-Santos A, et al.Differential tumor expression of inhibitor of differentiation-1 in prostate cancer patients with extreme clinical phenotypes and prognostic implications[J]. Clin Genitourin Cancer, 2014, 12(2): 87-93.
[6] Kubelac MP, Fetica B, Vlad IC, et al.The role of inhibitor of DNA-binding 1 (Id--1) protein and angiogenesis in serous ovarian cancer[J]. Anticancer Res, 2014, 34(1): 413-416.
[7] Zhou JD, Yang L, Zhu XW, et al.Clinical significance of up-regulated Id-1 expression in Chinese de novo acute myeloid leukemia[J]. Int J Clin Exp Pathol, 2015, 8(5): 5336-5344.
[8] Iliakis G, Krieg T, Guan J, et al.Evidence for an S-phase checkpoint regulating DNA replication after heat shock: a review[J]. Int J Hyperthermia, 2004, 20(2): 240-249.
[9] Yunoki T, Kariya A, Kondo T, et al.The combination of silencing BAG3 and inhibition of the JNK pathway enhances hyperthermia sensitivity in human oral squamous cell carcinoma cells[J]. Cancer Lett, 2013, 335(1): 52-57.
[10] Toraya-Brown S, Sheen MR, Zhang P, et al.Local hyperthermia treatment of tumors induces CD8(+) T cell-mediated resistance against distal and secondary tumors[J]. Nanomedicine, 2014, 10(6): 1273-1285.
[11] Dayanc BE, Bansal S, Gure AO, et al.Enhanced sensitivity of colon tumour cells to natural killer cell cytotoxicity after mild thermal stress is regulated through HSF1-mediated expression of MICA[J]. Int J Hyperthermia, 2013, 29(5): 480-490.
[12] Richter K, Haslbeck M, Buchner J.The heat shock response: life on the verge of death[J]. Mol Cell, 2010, 40(2): 253-266.
[13] Akerfelt M, Morimoto RI, Sistonen L.Heat shock factors: integrators of cell stress, development and lifespan[J]. Nat Rev Mol Cell Biol, 2010, 11(8): 545-555.
[14] Kim HY, Kim YS, Yun HH, et al.ERK-mediated phosphorylation of BIS regulates nuclear translocation of HSF1 under oxidative stress[J]. Exp Mol Med, 2016, 48(9): e260.
[15] Shah SP, Nooka AK, Jaye DL, et al.Bortezomib-induced heat shock response protects multiple myeloma cells and is activated by heat shock factor 1 serine 326 phosphorylation[J]. Oncotarget, 2016, 7(37): 59727-59741.
[16] Lasorella A, Benezra R, Iavarone A.The ID proteins: master regulators of cancer stem cells and tumour aggressiveness[J]. Nat Rev Cancer, 2014, 14(2): 77-91.
[17] Chen KY, Chen CC, Tseng YL, et al.GCIP functions as a tumor suppressor in non-small cell lung cancer by suppressing Id-1-mediated tumor promotion[J]. Oncotarget, 2014, 5(13): 5017-5028.
[18] Shin DH, Park JH, Lee JY, et al.Overexpression of Id-1 in transgenic mice promotes mammary basal stem cell activity and breast tumorigenesis[J]. Oncotarget, 2015, 6(19): 17276-17290.
[19] 武雪亮, 王立坤, 杨东东, 等. siRNA干扰Id--1表达对人结肠癌SW480细胞株生长与侵袭能力影响研究[J]. 中华肿瘤防治杂志, 2017, 24(3): 156-160.
[20] Gumireddy K, Li A, Kossenkov AV, et al.Id-1 promotes breast cancer metastasis by S100A9 regulation[J]. Mol Cancer Res, 2014, 12(9): 1334-1343.
[21] Ma H, Wei Y, Leng Y, et al.TGF-beta1-induced expression of Id-1 is associated with tumor progression in gastric cancer[J]. Med Oncol, 2014, 31(7): 19.
[22] Jin H, Xie X, Hu B, et al.Hyperthermia inhibits the proliferation and invasive ability of mouse malignant melanoma through TGF-β₍₁)[J]. Oncol Rep, 2013, 29(2): 725-734.
[23] Wang L, Man N, Sun XJ, et al.Regulation of AKT signaling by Id-1 controls t(8;21) leukemia initiation and progression[J]. Blood, 2015, 126(5): 640-650.
[24] Man J, Shoemake JD, Ma T, et al.Hyperthermia sensitizes glioma stem-like cells to radiation by inhibiting AKT signaling[J]. Cancer Res, 2015, 75(8): 1760-1769.
[25] Jiang S, Tu K, Fu Q, et al.Multifaceted roles of HSF1 in cancer[J]. Tumour Biol, 2015, 36(7): 4923-4931.