Expression and significance of ribosomal protein L29 in tongue squamous cell carcinoma

doi:10.19438/j.cjoms.2021.02.003

Abstract

Abstract: PURPOSE: To investigate the expression and significance of ribosomal protein L29 (RPL29) in human tongue squamous cell carcinoma (TSCC). METHODS: SP method of immunohistochemistry was carried out to detect the expression of RPL29 in cancer tissues and adjacent tissues of 52 TSCC patients. CAL-27 cells were transfected with RPL29-siRNA. RPL29 protein and mRNA levels were detected using Western blot and RT-PCR. Transwell invasion assay was performed to assess the invasion ability of CAL-27 cells. SPSS 21.0 software package was used for statistical analysis. RESULTS: The expression of RPL29 in TSCC was significantly higher than that in paracancerous tissues. RPL29 over-expression was found to be associated with cervical lymph node metastasis（P<0.05）,but not with age,gender,smoking history,alcohol drinking history, pathological type and T stage(P>0.05). After transfecting RPL29-siRNA into CAL-27 cells, the ability of invasion was significantly decreased. CONCLUSIONS: The expression of RPL29 in TSCC was significantly increased and its expression level was significantly different from that in patients with or without lymph node metastasis. The invasive ability of CAL-27 cells was significantly inhibited after transfection with RPL29-siRNA. RPL29 may play an important role in the processes of invasion and metastasis of TSCC.

Key words: Ribosomal protein L29, Tongue squamous cell carcinoma, RNA interference, Invasion

CLC Number:

R739.8

KONG Xian-chen, JIANG Xiao-dong, LU Xin, YU Wen-yi, XUE Jiao, ZHANG Peng, ZHAO Lu, YUAN Rong-tao. Expression and significance of ribosomal protein L29 in tongue squamous cell carcinoma[J]. China Journal of Oral and Maxillofacial Surgery, 2021, 19(2): 110-115.

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