Expression and clinical significance of MCM7 and HPV in squamous cell carcinoma of the maxillary sinus

doi:10.19438/j.cjoms.2017.05.003

Abstract

Abstract: PURPOSE: This study was designed to investigate the expression of minichromosome maintenance protein 7(MCM7) and human papillomavirus (HPV) in squamous cell carcinoma of the maxillary sinus (MSSCC) and its relationship with the pathogenesis of this disease and clinical significance. METHODS: Using immunohistochemistry method, we studied the expression of MCM7 and HPV in MSSCC (experimental group), corresponding adjacent tissues (adjacent group) and inferior turbinate mucosal tissues (control group). SPSS 22.0 software package was used to analyze the data. RESULTS: MCM7 was located in the basal layer of the epithelium in the adjacent group and the control group, but diffusedly distributed in the MSSCC. The expression of MCM7 in control (P=0.000), adjacent (P=0.000) and experimental (P=0.000) group increased gradually. The expression of MCM7 was only related to the degree of tumor differentiation (P=0.030), and the expression of MCM7 increased gradually with the decrease of the degree of differentiation. The cells infected with HPV had no obvious regularity in the control, the adjacent or the experimental group. The infection rates of HPV in the control group， the adjacent group and the experimental group were 40.00%（4/10）,95.65%（22/23）,78.57%（33/42）, respectively. HPV infection was associated with cervical lymph node metastasis (P=0.015), but not gender (P=1.000), age (P=0.332), smoking (P=0.536), clinical stage (P=0.806) and tumor differentiation (P=0.489). The expression level of MCM7 in patients with HPV infection was significantly higher than that in the uninfected patients (P=0.007), and there was a positive correlation between them (rs=0.317, P=0.041). CONCLUSIONS: MCM7 and HPV maybe correlate with the occurrence and development of MSSCC, which may have important significance for clinical treatment and prediction of prognosis.

Key words: Maxillary sinus, Squamous cell carcinoma, Minichromosome maintenance protein 7, Human papillomavirus

CLC Number:

R739.8 [文献标志码] A

HU Yue, LU Li, JIANG Fei-fei, DING Xiao-xu, WANG Jia-shuo, YAN Ai-hui.. Expression and clinical significance of MCM7 and HPV in squamous cell carcinoma of the maxillary sinus[J]. China Journal of Oral and Maxillofacial Surgery, 2017, 15(5): 392-396.

References

[1] Barnes L, Eveson JW, Reichart P, et al. World Health Organization classification of tumours. Pathology and ｇenetics of head and neck tumours [M].Lyon: IARC, 2005: 13.
[2] You Z, Ishimi Y, Masai H, et al. Roles of Mcm7 and Mcm4 subunits in the DNA helicase activity of the mouse Mcm4/6/7 complex [J]. J Biol Chem, 2002, 277(45): 42471-42479.
[3] Choy B, LaLonde A, Que J, et al. MCM4 and MCM7, potential novel proliferation markers, significantly correlated with Ki67, Bmi1, and cyclin E expression in esophageal adenocarcinoma, squamous cell carcinoma, and precancerous lesions [J]. Hum Pathol, 2016, 57: 126-135.
[4] Ishibashi Y, Kinugasa T, Akagi Y, et al. Minichromosome maintenance protein 7 is a risk factor for recurrence in patients with Dukes C colorectal cancer [J]. Anticancer Res, 2014, 34(8): 4569-4575.
[5] Nowinska K, Chmielewska M, Piotrowska A, et al. Correlation between levels of expression of minichromosome maintenance proteins, Ki-67 proliferation antigen and metallothionein I/II in laryngeal squamous cell cancer [J]. Int J Oncol, 2016, 48(2): 635-645.
[6] Laco J, Sieglová K, Vo?觢miková H, et al. The presence of high-risk human papillomavirus (HPV) E6/E7 mRNA transcripts in a subset of sinonasal carcinomas is evidence of involvement of HPV in its etiopathogenesis [J]. Virchows Arch, 2015, 467(4): 405-415.
[7] Xu W, Liu Z, Bao Q, et al. Viruses, other pathogenic microorganisms and esophageal cancer [J]. Gastrointest Tumors, 2015, 2(1):2-13.
[8] Galloway DA, Laimins LA. Human papillomaviruses: shared and distinct pathways for pathogenesis [J].Curr Opin Virol, 2015, 14: 87-92.
[9] Edge S, Byrd DR, Compton CC, et al. Cancer Staging Manual [M].7th Ed. New York: Springer, 2010: 57-67.
[10] Alos L, Moyano S, Nadal A, et al. Human papillomaviruses are identified in a subgroup of sinonasal squamous cell carcinomas with favorable outcome [J].Cancer, 2009, 115(12): 2701-2709.
[11] 李蕙君, 路晓薇, 李春阳, 等. 口腔鳞癌和癌前病变中微小染色体维持蛋白7和细胞分裂周期蛋白6的表达研究 [J]. 中华口腔医学研究杂志(电子版), 2010, 4(3): 224-231.
[12] Coli A, Asa SL, Fadda G, et al. Minichromosome maintenance protein 7 as prognostic marker of tumor aggressiveness in pituitary adenoma patients [J]. Eur J Endocrinol, 2016, 174(3): 307-314.
[13] Gray ST, Herr MW, Sethi RK, et al. Treatment outcomes and prognostic factors, including human papillomavirus, for sinonasal undifferentiated carcinoma: a retrospective review [J]. Head Neck, 2015, 37(3): 366-374.
[14] El-Mofty SK, Lu DW. Prevalence of high-risk human papillomavirus DNA in nonkeratinizing (cylindrical cell) carcinoma of the sinonasal tract: a distinct clinicopathologic and molecular disease entity [J]. Am J Surg Pathol, 2005, 29(10): 1367-1372.
[15] Malinowski DP. Molecular diagnostic assays for cervical neoplasia: emerging markers for the detection of high-grade cervical disease [J]. Biotechniques, 2005, Suppl: 17-23.
[16] Strati K, Pitot HC, Lambert PF. Identification of biomarkers that distinguish human papillomavirus (HPV)-positive versus HPV-negative head and neck cancers in a mouse model [J]. Proc Natl Acad Sci USA, 2006, 103(38): 14152-14157.