中国口腔颌面外科杂志

中国口腔颌面外科杂志 ›› 2020, Vol. 18 ›› Issue (6): 490-494.doi: 10.19438/j.cjoms.2020.06.004

• 论著 • 上一篇    下一篇

α桐酸对舌鳞状细胞癌CAL-27细胞的体外抑制作用

王子昱1, 丁宇洁1, 陈冰2, 沈昊翀3, 孟箭1,4,*, 仝伟凤1,*   

  1. 1.徐州医科大学 口腔医学院,江苏 徐州 221004;
    2.徐州医科大学 医学影像学院,江苏 徐州 221004;
    3.徐州医科大学 公共卫生学院,江苏 徐州 221004;
    4.徐州医科大学 徐州临床学院 口腔科,江苏 徐州 221009
  • 收稿日期:2020-05-21 修回日期:2020-07-20 发布日期:2020-12-31
  • 通讯作者: 孟箭,E-mail:mrocket@126.com;仝伟凤,E-mail:tongweifeng0320@foxmial.com。*共同通信作者
  • 作者简介:王子昱(1998-),女,学士,E-mail:kry1212@163.com
  • 基金资助:
    江苏省卫生计生委科研课题(H2017080);徐州市科技局重点研发项目(KC17196);江苏省高等学校大学生创新创业训练计划项目(201810313007Z);基础医学国家级实验教学示范中心(徐州医科大学)资助项目

Inhibitory effect of α-eleostearic acid on tongue squamous cell carcinoma CAL-27

WANG Zi-yu1, DING Yu-jie1, CHEN Bing2, SHEN Hao-chong3, MENG Jian4, TONG Wei-feng1   

  1. 1. School of Stomatology, Xuzhou Medical University. Xuzhou 221004;
    2. School of Medical Imaging, Xuzhou Medical University. Xuzhou 221004;
    3. School of Public Health, Xuzhou Medical University. Xuzhou 221004;
    4. School of Clinical Medicine, Xuzhou Medical University. Xuzhou 221009, Jiangsu Province, China
  • Received:2020-05-21 Revised:2020-07-20 Published:2020-12-31

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摘要： 目的: 探讨苦瓜籽油中的α桐酸(α-eleostearic acid,α-ESA)对舌鳞状细胞癌CAL-27细胞增殖、迁移、凋亡的影响。方法: 将0、70、80、90、100、110、120 μmol/L的α-ESA培养CAL-27细胞后,用CCK-8法和流式细胞术分别检测加药24、48、72 h后细胞增殖抑制率和加药48 h后细胞凋亡率。再分别以0、80、100 μmol/L的α-ESA培养CAL-27细胞不同时间后,用细胞划痕实验检测加药24 h后细胞的迁移能力,Hoechst 33258染色在荧光显微镜下观察加药48 h后凋亡细胞形态。采用SPSS 21.0软件包对实验数据进行统计学分析。结果: α-ESA对CAL-27细胞的增殖抑制作用呈明显时间和浓度依赖性(P<0.05)。作用24、48、72 h后,半数抑制浓度(IC50)逐渐降低,分别为(123.48±1.00)、(80.22±0.03)和(69.27±80.34) μmol/L。流式细胞检测结果显示,细胞凋亡率随着α-ESA浓度的增加而大幅增高(P<0.05)。培养24 h后,加入80、100 μmol/L的α-ESA的细胞划痕愈合率分别为(40.08±2.19)%、(22.06±2.04)%,显著低于对照组(74.74±2.17)%(P<0.01)。荧光显微镜下观察到致密浓染或碎块状亮蓝色荧光的细胞凋亡现象。结论: α-ESA能够抑制CAL-27细胞的体外增殖及迁移能力,并可诱导细胞凋亡,从而产生一定的抗肿瘤生长作用。

关键词: 苦瓜籽油, α桐酸, α-ESA, 舌鳞状细胞癌, CAL-27, 细胞增殖, 迁移, 凋亡

Abstract: PURPOSE: To investigate the effect of α-eleostearic acid (α-ESA) in bitter melon seed oil on the proliferation, migration and apoptosis of tongue squamous cell carcinoma CAL-27 cells. METHODS: CAL-27 cells were cultivated in vitro and α-ESA was added at the concentrations of 0, 70, 80, 90, 100, 110 and 120 μmol/L. CCK-8 assay and flow cytometry were used to detect the cell proliferation inhibition rate at 24, 48, and 72 h and apoptosis rate at 48 h respectively. CAL-27 cells were treated with α-ESA at concentrations of 0, 80 and 100 μmol/L. The cell migration capacity after 24 hours of α-ESA administration was measured by cell scratching assay. The morphology of apoptotic cells after 48 h of α-ESA administration was observed by Hoechst 33258 staining. SPSS 21.0 software package was used for statistical analysis. RESULTS: The proliferation of CAL-27 cells was inhibited by α-ESA in a time and concentration-dependent manner (P<0.05). After 24, 48, and 72 h of α-ESA administration, the half inhibitory concentration(IC50) was (123.48±1.00) μmol/L, (80.22±0.03) μmol/L, and (69.27±80.34) μmol/L, respectively, which was gradually decreased. The results of flow cytometry showed that the apoptosis rate increased significantly with the increase of α-ESA concentration (P<0.05). After 24 h of incubation, the wound healing rate of CAL-27 cells was (40.08±2.19)% and (22.06±2.04)% after administration of 80 and 100 μmol/L α-ESA, which was significantly lower than the control group (74.74±2.17)%(P<0.01). Densely stained or fragmented bright blue fluorescence was observed under fluorescence microscope which represented cell apoptosis. CONCLUSIONS: α-ESA can inhibit the proliferation and migration of CAL-27 cells in vitro, and therefore has certain anti-tumor effects.

Key words: α-eleostearic acid, α-ESA, Bitter melon seed oil, Tongue squamous cell carcinoma, Cal-27 cell, Cell proliferation, Migration, Apoptosis

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