SREBP2对口腔鳞癌细胞增殖和迁移的影响及其调控机制

doi:10.19438/j.cjoms.2021.04.002

中国口腔颌面外科杂志 ›› 2021, Vol. 19 ›› Issue (4): 295-301.doi: 10.19438/j.cjoms.2021.04.002

SREBP2对口腔鳞癌细胞增殖和迁移的影响及其调控机制

郭铭^1,2, 陈羽菱², 吴利², 张璧茹², 沈月洪^2,*, 杨宏宇^2,*

1.北京大学口腔医学院·口腔医院综合二科,国家口腔疾病临床医学研究中心, 口腔数字化医疗技术和材料国家工程实验室,口腔数字医学北京市重点实验室,北京 100081;
2.北京大学深圳医院口腔医学中心口腔颌面外科,广东省高水平临床重点专科, 广东省口腔疾病诊疗技术工程技术研究中心,广东深圳 518036

收稿日期:2020-12-17 修回日期:2021-03-03 出版日期:2021-07-20 发布日期:2021-08-05
通讯作者: 沈月洪,E-mail: yuehongshen@hotmail.com ; 杨宏宇,E-mail: hyyang192@hotmail.com。^*共同通信作者
作者简介:郭铭（1994-）,女,硕士研究生,E-mail：erapac@live.cn
基金资助:
广东省基础与应用基础研究基金（2019A1515011911）; 深圳市科技创新基础研究基金（JCYJ20200109140208058、JCYJ20190809104803572）; 广东省高水平临床重点专科（深圳市配套建设经费,SZGSP008）

Effect of SREBP2 on proliferation and migration of oral squamous cell carcinoma and its regulatory mechanism

GUO Ming^1,2, CHEN Yu-ling², WU Li², ZHANG Bi-ru², SHEN Yue-hong², YANG Hong-yu²

1. Second Department of General Dentistry, Peking University School of Stomatology, National Clinical Research Center for Oral Diseases, National Engineering Laboratory of Dental Digital Medical Technology and Materials, Beijing Key Laboratory of Oral Digital Medicine. Beijing 100081;
2. Department of Oral and Maxillofacial Surgery, Stomatological Center, Peking University Shenzhen Hospital, Guangdong Provincial High-level Clinical Key Specialty, Guangdong Province Engineering Research Center of Oral Disease Diagnosis and Treatment. Shenzhen 518036, Guangdong Province, China

Received:2020-12-17 Revised:2021-03-03 Online:2021-07-20 Published:2021-08-05

摘要/Abstract

摘要： 目的： 探讨胆固醇调节元件结合蛋白2（SREBP2）在口腔鳞癌（OSCC）中的调控机制,通过敲低和过表达SREBP2,分析其对口腔鳞癌细胞CAL27增殖、迁移、侵袭的影响及作用机制。方法： 采用实时定量反转录PCR（qRT-PCR）检测SREBP2在OSCC中的表达量。构建siRNA和过表达质粒并转染 CAL27细胞,通过细胞计数（CCK-8）实验和EdU染色检测细胞增殖能力,Annexin V-FITC/PI双染实验检测细胞凋亡水平,划痕愈合和Transwell实验检测细胞迁移、侵袭能力,通过蛋白免疫印迹法检测SREBP2调控MVA通路相关蛋白表达的改变。数据使用Graphpad 5.0软件绘图,组间比较采用t检验。结果： SREBP2在50对口腔鳞癌组织和OSCC细胞系中表达较正常对照组显著降低。敲低及过表达实验表明,SREBP2表达的变化影响OSCC细胞的增殖、迁移、侵袭,并通过MVA信号通路影响OSCC的发展。结论： SREBP2在OSCC中抑制癌细胞增殖、迁移、侵袭并促进凋亡。

关键词: 胆固醇调节元件结合蛋白2, 口腔鳞癌, MVA细胞通路, 增殖, 迁移

Abstract: PURPOSE: To investigate the effect of sterol regulatory element-binding protein 2 (SREBP2) on the malignant biological behaviors of oral squamous cell carcinoma (OSCC) cell lines. METHODS: The expression of SREBP2 in OSCC was detected by qRT-PCR. SiRNA and overexpression plasmids were constructed and transferred into CAL27. The ability of cell proliferation was detected by cell counting kit-8(CCK-8) assay and EdU cell-proliferation assay; the level of apoptosis was detected by flow cytometry; the ability of migration and invasion was detected by wound-healing assay and Transwell migration and invasion assays. Western blotting was used to detect the expression of SREBP2-related proteins. The data were plotted by Graphpad 5.0; Student's t tests were used to evaluate difference among groups. RESULTS: The expression of SREBP2 in 50 pairs of OSCC tissues and OSCC cell lines was significantly lower than that in normal controls. Knock-down and overexpression experiments showed that the change of SREBP2 expression affected the malignant biological behavior of OSCC cells and affected the development of OSCC through MVA signal pathway. CONCLUSIONS: SREBP2 inhibits proliferation, migration, invasion and promotes apoptosis of cancer cells in OSCC.

Key words: SREBP2, Oral squamous cell carcinoma, MVA signaling pathway, Proliferation, Migration

中图分类号:

R739.8

郭铭, 陈羽菱, 吴利, 张璧茹, 沈月洪, 杨宏宇. SREBP2对口腔鳞癌细胞增殖和迁移的影响及其调控机制[J]. 中国口腔颌面外科杂志, 2021, 19(4): 295-301.

GUO Ming, CHEN Yu-ling, WU Li, ZHANG Bi-ru, SHEN Yue-hong, YANG Hong-yu. Effect of SREBP2 on proliferation and migration of oral squamous cell carcinoma and its regulatory mechanism[J]. China J Oral Maxillofac Surg, 2021, 19(4): 295-301.

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SREBP2对口腔鳞癌细胞增殖和迁移的影响及其调控机制

Effect of SREBP2 on proliferation and migration of oral squamous cell carcinoma and its regulatory mechanism

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