基质金属蛋白酶9抑制剂对人舌鳞癌SCC-25细胞侵袭、迁移能力的影响及机制探讨

doi:10.19438/j.cjoms.2021.05.007

中国口腔颌面外科杂志 ›› 2021, Vol. 19 ›› Issue (5): 424-428.doi: 10.19438/j.cjoms.2021.05.007

基质金属蛋白酶9抑制剂对人舌鳞癌SCC-25细胞侵袭、迁移能力的影响及机制探讨

水文军¹, 高金星¹, 任蕾²

1.浙江省人民医院口腔科,浙江杭州 310014;
2.杭州美莱医疗美容医院口腔科,浙江杭州 310005

收稿日期:2021-01-14 修回日期:2021-04-01 出版日期:2021-09-20 发布日期:2021-10-20
通讯作者: 任蕾,E-mail:652276717@qq.com
作者简介:水文军（1978-）,男,本科,主治医师,E-mail:78swj@163.com

Effect of MMP-9 inhibitor on invasion and migration ability of human tongue squamous cell carcinoma SCC-25 cell and its mechanism

SHUI Wen-jun¹, GAO Jin-xing¹, REN Lei²

1. Department of Stomatology, Zhejiang Provincial People’s Hospital. Hangzhou 310014, Zhejiang Province;
2. Department of Stomatology, Hangzhou Mylike Cosmetic Hospital. Hangzhou 310005, Zhejiang Province, China

Received:2021-01-14 Revised:2021-04-01 Online:2021-09-20 Published:2021-10-20

摘要/Abstract

摘要： 目的探讨基质金属蛋白酶9（MMP-9）抑制剂对人舌鳞状细胞癌（TSCC）SCC-25细胞侵袭、迁移能力的影响及其相关机制。方法取对数生长期SCC-25细胞,分别采用0、5、10、20 μmol/L的MMP-9抑制剂BB-94干预,分别设为对照组、实验A组、实验B组和实验C组。采用RT-qPCR和Western免疫印迹测定MMP-9 mRNA及蛋白的表达;Transwell小室实验检测细胞侵袭能力;划痕实验测定迁移能力;Western免疫印迹测定细胞间黏附分子-1（ICAM-1）、E钙黏附蛋白（E-cad）、间隙连接蛋白43（Cx43）的蛋白表达水平。采用SPSS 22.0软件包对数据进行统计学分析。结果与对照组相比,实验A、B、C组MMP-9 mRNA及蛋白相对表达量、迁移率降低,穿膜细胞数量/视野减少（P<0.05）;与实验A组相比,实验B、C组MMP-9 mRNA及蛋白相对表达量、迁移率降低,穿膜细胞数量/视野减少（P<0.05）;与实验B组相比,实验C组MMP-9 mRNA及蛋白相对表达量、迁移率降低,穿膜细胞数量/视野减少（P<0.05）。与对照组相比,实验A、B、C组ICAM-1蛋白相对表达量降低,E-cad、Cx43蛋白相对表达量升高（P<0.05）;与实验A组相比,实验B、C组ICAM-1蛋白相对表达量降低,E-cad、Cx43蛋白相对表达量升高（P<0.05）;与实验B组相比,实验C组ICAM-1蛋白相对表达量降低,E-cad、Cx43蛋白相对表达量升高（P<0.05）。结论 MMP-9抑制剂可抑制TSCC细胞侵袭、迁移,且呈剂量依赖性,推测其作用机制与下调ICAM-1表达,上调E-cad、Cx43表达有关。

关键词: 人舌鳞状细胞癌, 侵袭, 迁移, 基质金属蛋白酶9

Abstract: PURPOSE: To study the effect of matrix metalloproteinase-9 (MMP-9) inhibitors on invasion and migration ability of human tongue squamous cell carcinoma (TSCC) SCC-25 cells, and explore its related mechanisms. METHODS: SCC-25 cells in the logarithmic phase were taken and treated with 0, 5, 10, and 20 μmol/L MMP-9 inhibitor BB-94 respectively, and divided into control group, experimental group A, B and C. RT-qPCR, Western blot were used to determine the expression of MMP-9. Transwell chamber experiment was used to determine invasion ability. Scratch test was used to determine migration ability. Western blot was used to determine the protein expression of intercellular adhesion molecule-1 (ICAM-1), E-cadherin (E-cad), and connexin 43 (Cx43). SPSS 22.0 software package was used for data analysis. RESULTS: Compared with the control group, the relative expression of MMP-9 mRNA and protein, migration rate, and number of penetrating cells/field of view in experimental A, B, and C group decreased significantly(P<0.05). Compared with experimental group A, the relative expression of MMP-9 mRNA and protein, migration rate, and number of penetrating cells/field of view in experimental B, and C group decreased significantly (P<0.05). Compared with experimental group B, the relative expression of MMP-9 mRNA and protein, migration rate, the number of penetrating cells/field of view in experimental group C decreased(P<0.05). Compared with the control group, the relative expression of ICAM-1 protein in experimental group A, B, and C decreased, while the relative expression of E-cad and Cx43 protein increased significantly (P<0.05). Compared with experimental group A, the relative expression of ICAM-1 protein in experimental group B and C decreased, while the relative expression of E-cad and Cx43 protein increased significantly (P<0.05). Compared with experimental group B, the relative expression of ICAM-1 protein in experimental group C decreased, while the relative expression of E-cad and Cx43 protein increased(P<0.05). CONCLUSIONS: MMP-9 inhibitor can inhibit TSCC cell invasion and migration in a dose-dependent manner. It is speculated that its mechanism of action is related to down-regulation of ICAM-1 expression and up-regulation of E-cad and Cx43 expression.

Key words: Human tongue squamous cell carcinoma, Invasion, Migration, Matrix metalloproteinase-9

中图分类号:

R739.8

水文军, 高金星, 任蕾. 基质金属蛋白酶9抑制剂对人舌鳞癌SCC-25细胞侵袭、迁移能力的影响及机制探讨[J]. 中国口腔颌面外科杂志, 2021, 19(5): 424-428.

SHUI Wen-jun, GAO Jin-xing, REN Lei. Effect of MMP-9 inhibitor on invasion and migration ability of human tongue squamous cell carcinoma SCC-25 cell and its mechanism[J]. China J Oral Maxillofac Surg, 2021, 19(5): 424-428.

参考文献

[1] Campbell BR, Sanders CB, Netterville JL, et al.Early onset oral tongue squamous cell carcinoma: associated factors and patient outcomes[J]. Head Neck, 2019, 41(6): 1952-1960.
[2] Yuan S, Lin LS, Gan RH, et al.Elevated matrix metalloproteinase 7 expression promotes the proliferation, motility and metastasis of tongue squamous cell carcinoma[J]. BMC Cancer, 2020, 20(1):33-44.
[3] Jafarian AH, Kooshki Forooshani M, Reisi H, et al.Matrix metalloproteinase-9 (MMP-9) expression in non-small cell lung carcinoma and its association with clinicopathologic factors[J]. Iran J Pathol, 2020, 15(4): 326-333.
[4] Ashraf TS, Obaid A, Saeed TM, et al.Formal model of the interplay between TGFβ1 and MMP-9 and their dynamics in hepatocellular carcinoma[J]. Math Biosci Eng, 2019, 16(5): 3285-3310.
[5] Väyrynen O, Äström P, Nyberg P, et al.Matrix metalloproteinase 9 inhibits the motility of highly aggressive HSC-3 oral squamous cell carcinoma cells[J]. Exp Cell Res, 2019, 376(1): 18-26.
[6] Ohta K, Yoshimura H.Squamous cell carcinoma of the dorsal tongue[J]. CMAJ, 2019, 191(47): E1310-E1310.
[7] Hararah MK, Stokes WA, Oweida A, et al.Epidemiology and treatment trends for primary tracheal squamous cell carcinoma[J]. Laryngoscope, 2020, 130(2): 405-412.
[8] Zhu L, Wang Y, Li R, et al.Surgical treatment of early tongue squamous cell carcinoma and patient survival[J]. Oncol Lett, 2019, 17(6): 5681-5685.
[9] Huang W, Zhou R, Mao L, et al.Esophageal cancer related gene-4 inhibits the migration and proliferation of oral squamous cell carcinoma through BC200 lncRNA/MMP-9 and -13 signaling pathway[J]. Cell Signal, 2019, 62: 109327.
[10] Karmakar D, Maity J, Mondal P, et al.E2F5 promotes prostate cancer cell migration and invasion through regulation of TFPI2, MMP-2 and MMP-9[J]. Carcinogenesis, 2020, 41(12): 1767-1780.
[11] Yuan J, Xu XJ, Lin Y, et al.LncRNA MALAT1 expression inhibition suppresses tongue squamous cell carcinoma proliferation, migration and invasion by inactivating PI3K/Akt pathway and downregulating MMP-9 expression[J]. Eur Rev Med Pharmacol Sci, 2019, 23(1): 198-206.
[12] Magrin GL, Di Summa F, Strauss FJ, et al.Butyrate decreases ICAM-1 expression in human oral squamous cell carcinoma cells[J]. Int J Mol Sci, 2020, 21(5): 1679-1693.
[13] Halpern AL, Kohtz PD, Rove JY, et al.Inhibition of secretory phospholipase A2 Ⅱa attenuates prostaglandin E2-induced invasiveness in lung adenocarcinoma[J]. Mol Cell Biochem, 2019, 456(1-2): 145-156.
[14] Yuan L, Zhou M, Wasan HS, et al.Jiedu sangen decoction inhibits the invasion and metastasis of colorectal cancer cells by regulating EMT through the hippo signaling pathway[J]. Evid Based Complement Alternat Med, 2019: 1431726.
[15] Song XH, Chen XZ, Chen XL, et al.Peritoneal metastatic cancer stem cells of gastric cancer with partial mesenchymal-epithelial transition and enhanced invasiveness in an intraperitoneal transplantation model[J]. Gastroenterol Res Pract, 2020: 3256538.
[16] Che X, Lu R, Fu Z, et al.Therapeutic effects of tyroserleutide on lung metastasis of human hepatocellular carcinoma SK-HEP-1 and its mechanism affecting ICAM-1 and MMP-2 and -9[J]. Drug Des Devel Ther, 2018, 12: 3357-3368.
[17] 程琦, 徐松涛, 饶淑梅, 等. 托芬那酸通过下调LOXL2表达抑制人胃癌SGC-7901细胞侵袭[J]. 中国免疫学杂志, 2020, 36(21): 2624-2634.
[18] Xie D, Zheng GZ, Xie P, et al.Antitumor activity of resveratrol against human osteosarcoma cells: a key role of Cx43 and Wnt/β-catenin signaling pathway[J]. Oncotarget, 2017, 8(67): 111419-111432.

基质金属蛋白酶9抑制剂对人舌鳞癌SCC-25细胞侵袭、迁移能力的影响及机制探讨

Effect of MMP-9 inhibitor on invasion and migration ability of human tongue squamous cell carcinoma SCC-25 cell and its mechanism

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