丙泊酚对人咽鳞癌FADu细胞系增殖、迁移、侵袭和凋亡的调节作用

doi:10.19438/j.cjoms.2021.03.003

中国口腔颌面外科杂志 ›› 2021, Vol. 19 ›› Issue (3): 201-204.doi: 10.19438/j.cjoms.2021.03.003

丙泊酚对人咽鳞癌FADu细胞系增殖、迁移、侵袭和凋亡的调节作用

王越, 劳蔚^*, 孙宇^*

上海交通大学医学院附属第九人民医院麻醉科,上海 200011

收稿日期:2020-12-12 修回日期:2021-01-16 发布日期:2021-07-16
通讯作者: 孙宇,E-mail: Dr_sunyu@163.com;劳蔚,E-mail：jackielao17@163.com。^*共同通信作者
作者简介:王越（1996-）,女,在读硕士研究生,E-mail: Damon_wy@126.com
基金资助:
上海市科学技术委员会资助项目（18ZR1422900）

Effects of propofol on proliferation, migration, invasion and apoptosis of human pharyngeal squamous cell carcinoma FADu cell line

WANG Yue, LAO Wei, SUN Yu

Department of Anesthesiology, Shanghai Ninth People's Hospital, Shanghai Jiao Tong University School of Medicine. Shanghai 200011, China

Received:2020-12-12 Revised:2021-01-16 Published:2021-07-16

摘要/Abstract

摘要： 目的探讨丙泊酚对人咽鳞癌FADu细胞系增殖、迁移、侵袭和促进凋亡的潜在机制。方法将不同浓度（0、5、10、20 μg/mL）丙泊酚处理的人咽鳞癌FADu细胞系分别进行CCK-8毒性测试,平板克隆实验测试增殖能力,划痕实验检测细胞横向迁移能力,Transwell实验检测细胞纵向迁移能力和侵袭能力,通过Western 免疫印迹实验检测Bcl-2、Bax、Cleaved Caspase-3蛋白表达情况。采用SPSS 18.0软件包对数据进行统计学分析。结果细胞毒性实验表明,人咽鳞癌FADu细胞系存活率在不同浓度丙泊酚处理后呈时间浓度依赖性下降（P<0.01）,随着处理时间增加,细胞存活率显著下降。平板克隆实验表明,细胞增殖能力随着丙泊酚浓度增高而显著下降（P<0.01）。划痕实验表明,10、20 μg/mL丙泊酚处理的细胞横向迁移能力与对照组有显著差异（P<0.05）。Transwell实验表明,丙泊酚有效抑制纵向迁移能力和侵袭能力（P<0.001）,且呈浓度依赖性。Western免疫印迹实验检测表明,Bcl-2表达递减,Bax、Cleaved Caspase-3表达递增（P<0.01）;随着丙泊酚处理浓度的增加,细胞显著凋亡。结论丙泊酚抑制人咽鳞癌细胞FADu增殖、迁移和侵袭,并且通过抑制Bcl-2/Bax通路促进凋亡,其作用机制可能是促进Caspase-3的表达。

关键词: 丙泊酚, FADu细胞系, 侵袭, 凋亡

Abstract: PURPOSE: To study the potential mechanism of propofol on proliferation, migration, invasion and apoptosis of human pharyngeal squamous cell carcinoma FADu cell line. METHODS: Different concentrations of propofol (0, 5, 10, 20 μg/mL) were used to treat FADu cell line. CCK-8 toxicity test and plate clone test were used to test cell proliferation ability. The ability of lateral migration was detected by wound healing assay. Transwell assay was used to detect the ability of longitudinal migration and invasion. The expression of Bcl-2,Bax and Cleaved Caspase-3 was detected by Western blot. SPSS 18.0 software package was used to analyze the data. RESULTS: Cytotoxicity test showed that the survival rate of FADu cell line decreased in a time and concentration dependent manner (P<0.01) after treatment with different concentrations of propofol. The results of plate clonal assay showed that cell proliferation decreased with the increase of propofol concentration(P<0.01). Wound healing assay showed that the lateral migration ability of cells treated with propofol of 10 and 20 μg/mL was significantly different from that of the control group (P<0.05). Transwell test showed that propofol effectively inhibited the ability of longitudinal migration and invasion (P<0.001) in a concentration dependent manner. Western blot analysis showed that the expression of Bcl-2 decreased and the expression of Bax and Cleaved Caspase-3 increased significantly(P<0.01). CONCLUSIONS: Propofol inhibits the proliferation, migration and invasion of FADu cells, and promotes apoptosis by inhibiting Bcl-2/Bax pathway. The mechanism may be through promoting the expression of Caspase-3.

Key words: Propofol, FADu, Invasion, Apoptosis

中图分类号:

R739.8

王越, 劳蔚, 孙宇. 丙泊酚对人咽鳞癌FADu细胞系增殖、迁移、侵袭和凋亡的调节作用[J]. 中国口腔颌面外科杂志, 2021, 19(3): 201-204.

WANG Yue, LAO Wei, SUN Yu. Effects of propofol on proliferation, migration, invasion and apoptosis of human pharyngeal squamous cell carcinoma FADu cell line[J]. China J Oral Maxillofac Surg, 2021, 19(3): 201-204.

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丙泊酚对人咽鳞癌FADu细胞系增殖、迁移、侵袭和凋亡的调节作用

Effects of propofol on proliferation, migration, invasion and apoptosis of human pharyngeal squamous cell carcinoma FADu cell line

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