β肾上腺素受体在舌癌CAL-27细胞系的表达及β受体阻滞剂对其增殖、迁移、侵袭的影响

doi:10.19438/j.cjoms.2018.05.002

中国口腔颌面外科杂志 ›› 2018, Vol. 16 ›› Issue (5): 391-396.doi: 10.19438/j.cjoms.2018.05.002

β肾上腺素受体在舌癌CAL-27细胞系的表达及β受体阻滞剂对其增殖、迁移、侵袭的影响

刘诗奇, 羊良慧, 陈国生, 王辉, 房芳, 廖显翔, 麦华明

广西医科大学附属口腔医院口腔颌面外科,广西南宁 530021

收稿日期:2018-05-23 出版日期:2018-09-20 发布日期:2018-11-06
通讯作者: 麦华明,E-mail：dddmai@126.com
作者简介:刘诗奇（1990-）,男,在读硕士研究生,E-mail：603387283@qq.com
基金资助:
国家自然科学基金（81460413）

The expression of beta adrenergic receptor in CAL-27 cell line of tongue carcinoma and the effects of beta blockers on proliferation, migration and invasion

LIU Shi-qi, YANG Liang-hui, CHEN Guo-sheng, WANG hui, FANG fang, LIAO Xian-xiang, MAI Hua-ming

Department of Oral and Maxillofacial Surgery, Hospital of Stomatology, Guangxi Medical University. Nanning 530021, Guangxi Zhuang Autonomous Region, China

Received:2018-05-23 Online:2018-09-20 Published:2018-11-06

摘要/Abstract

摘要： 目的: 研究舌癌CAL-27细胞系中β-AR的表达及β受体阻滞剂对其增殖、迁移、侵袭的影响。方法: 采用Pan CK抗体对培养的正常口腔上皮细胞进行鉴定;免疫组织化学法及qRT-PCR法检测β-AR在舌癌CAL-27细胞的表达;CCK-8法、细胞划痕实验、Transwell法分别检测普萘洛尔及美托洛尔对CAL-27细胞增殖、迁移、侵袭的影响。采用SPSS16.0软件包对数据进行统计学分析。结果: β1-AR、β2-AR在CAL-27细胞的表达均显著高于正常口腔上皮细胞（P<0.05）;β2-AR在CAL-27细胞的mRNA水平表达低于正常口腔上皮细胞而β1-AR表达高于正常口腔上皮细胞;两种药物对CAL-27细胞增殖均有抑制作用,药物浓度>50 μmol/L时,普萘洛尔抑制增殖的作用更强（P<0.05）;2种药物浓度>2.5 μmol/L,对CAL-27细胞迁移均有抑制作用,普萘洛尔的抑制作用更强;药物浓度>1.0 μmol/L,2种药物对CAL-27细胞侵袭均有抑制作用（P<0.05）,普萘洛尔对CAL-27细胞迁移抑制作用更强。结论: 在舌癌CAL-27细胞中, β1-AR在mRNA水平的表达高于正常口腔上皮细胞而β2-AR的表达低于正常口腔上皮细胞;低浓度普萘洛尔及美托洛尔对CAL-27细胞的增殖无明显抑制作用,但能抑制CAL-27的迁移和侵袭。

关键词: β肾上腺素受体, CAL-27细胞系, 美托洛尔, 普萘洛尔

Abstract: PURPOSE: To investigate the expression of β adrenergic receptors in CAL-27 cell line of tongue carcinoma and the effects of β-blockers on the proliferation , migration and invasion of CAL-27 cells. METHODS: Normal oral mucosal epithelial cells (NOMEC) were cultured and identified. Immunohistochemistry and real-time quantitative PCR(qRT-PCR) were applied to detect the expression of β-adrenergic receptor in CAL-27 and oral mucosal epithelial cells. The proliferation, migration and invasion of CAL-27 cells were investigated by CCK-8 method, scratch adhesion test and Transwell assay under different concentrations of propranolol and metoprolol. Experimental data were analyzed with analysis of variance and t test by SPSS 16.0 software package. RESULTS: The expression of β1-AR and β2-AR in CAL-27 cells were higher than that in NOMEC, while the expression of β2-AR at mRNA level were lower than that in NOMEC, the expression of β1-AR was higher than that in NOMEC. CCK-8 assay showed with the increase of drug concentration, the inhibitory effect on CAL-27 cell proliferation was enhanced. When the drug concentration was >50 μmol/L, the inhibiting effect of propranolol on cell proliferation was significant (P<0.05). Scratch-wound assay showed that when drug concentration was >2.5 μmol/L, migration of CAL-27 cells was inhibited (P<0.05), and propranolol had a greater inhibitive effect. Transwell assay showed that when drug concentration >1.0 μmol/L,invasion of CAL-27 cells was suppressed (P<0.05), the suppression effect of propranolol was greater. CONCLUSIONS: The expression of β1-AR in CAL-27 cells is significantly higher than that in NOMEC and the expression of β2-AR is lower than that in NOMEC. Low concentration of propranolol and metoprolol has no significant effect on proliferation of CAL-27 cells, but can inhibit the migration and invasion of CAL-27 cells.

Key words: β-AR, CAL-27 cells, Metoprolol, Propranolol

中图分类号:

R739.8

刘诗奇, 羊良慧, 陈国生, 王辉, 房芳, 廖显翔, 麦华明. β肾上腺素受体在舌癌CAL-27细胞系的表达及β受体阻滞剂对其增殖、迁移、侵袭的影响[J]. 中国口腔颌面外科杂志, 2018, 16(5): 391-396.

LIU Shi-qi, YANG Liang-hui, CHEN Guo-sheng, WANG hui, FANG fang, LIAO Xian-xiang, MAI Hua-ming. The expression of beta adrenergic receptor in CAL-27 cell line of tongue carcinoma and the effects of beta blockers on proliferation, migration and invasion[J]. China J Oral Maxillofac Surg, 2018, 16(5): 391-396.

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β肾上腺素受体在舌癌CAL-27细胞系的表达及β受体阻滞剂对其增殖、迁移、侵袭的影响

The expression of beta adrenergic receptor in CAL-27 cell line of tongue carcinoma and the effects of beta blockers on proliferation, migration and invasion

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