Effect of RAC1 on migration and invasion of human squamous cell carcinoma of tongue cell line of CAL27

doi:10.19438/j.cjoms.2018.02.003

Abstract

Abstract: PURPOSE: To investigate the effect of RAC1 on invasion and migration of human squamous cell carcinoma of tongue cells of CAL27, as well as the possible molecular mechanism. METHODS: The specific small interfering RNAs (siRNAs) was designed and constructed on the basis of RAC1 gene sequence. Through liposome mediation, these three RAC1 siRNAs were transfected into CAL27 cells to inhibit RAC1 expression. Meanwhile, negative control group and blank control group were transfected with random sequence NC-siRNA and liposome, respectively. The mRNA of RAC1 were detected by real-time fluorescence quantitative polymerase chain reaction and the protein expression of RAC1, PAK1, LIMK1 were examined by Western blotting. The migration and invasion ability were analyzed by wound healing assay and Transwell chamber test. One-Way ANOVA and student's t test were used for data analysis with SPSS 18.0 software package. RESULTS: The protein expression of RAC1 was decreased significantly 48h after transfection (P<0.05), the expression of PAK1 and LIMK1 were suppressed (P<0.05), the migration and invasion ability of CAL27 cells was also significantly inhibited (P<0.05). CONCLUSIONS: Silencing RAC1 gene can inhibit migration and invasion ability of human squamous cell carcinoma of tongue CAL27 cells, and the mechanism may be related to down-regulation of PAK1 and LIMK1 expression in tongue cancer cells.

Key words: RAC1, siRNA, Tongue, Squamous cell carcinoma

CLC Number:

R739.8

HE Zong-xuan, WANG Wei, WANG Qi-min, TONG Lei, SHENG Shan-gui, CHEN Zheng-gang. Effect of RAC1 on migration and invasion of human squamous cell carcinoma of tongue cell line of CAL27[J]. China Journal of Oral and Maxillofacial Surgery, 2018, 16(2): 108-113.

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