Expression of BDNF/TrkB in the progress of epithelial-mesenchymal transition in salivary adenoid cystic carcinoma

Abstract

Abstract: PURPOSE: To investigate the expression of BDNF,TrkB and E-cadherin in salivary adenoid cystic carcinoma (SACC) and study their role in metastasis and nerve invasion. METHODS: The high and low metastatic SACC cell lines (SACC-LM, SACC-83) were analyzed by real-time PCR and Western blot to determine the expression of BDNF, TrkB and E-cadherin. To observe the BDNF/TrkB pathway in the metastasis process of SACC, the SACC-83 cells were treated by exogenous BDNF and/or TrkB inhibitor k252a, respectively. Meanwhile, the epithelial mesenchymal transition (EMT) of SACC-83 cells were evaluated by real-time PCR, Western blot, morphology observation, cell migration and invasion analysis. SPSS 17.0 software package was employed to analyze the data statistically. RESULTS: The expression of BDNF and TrkB in SACC-LM cells were higher than SACC-83 cells whereas the E-cadherin was down regulated. Exogenous BDNF could activate TrkB, inhibit E-cadherin and promote N-cadherin expression in SACC-83 cells. The morphology of SACC-83 cells stimulated by BDNF was also elongated and migration and invasion capabilities were improved. When TrkB was inhibited by k252a, the BDNF effects were inhibited including the suppressing of SACC-83 morphology alteration, migration and invasion capabilities. CONCLUSIONS: BDNF/TrkB pathway can promote the migration and invasion capabilities of SACC cells by mediating its EMT progression.

Key words: BDNF, TrKB, E-cadherin, Salivary adenoid cystic carcinoma, Tumor invasion, Epithelial-mesenchymal transition, EMT

CLC Number:

R739.87

HU Zhi-qiang, YANG Xin-jie, WANG Wei-xi, JIA Sen, WU Bao-lei, LEI De-lin. Expression of BDNF/TrkB in the progress of epithelial-mesenchymal transition in salivary adenoid cystic carcinoma[J]. China Journal of Oral and Maxillofacial Surgery, 2015, 13(3): 211-215.

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