The effect of NDRG2 on proliferation and invasion of salivary adenoid cystic carcinoma

doi:10.19438/j.cjoms.2019.02.003

Abstract

Abstract: PURPOSE: To investigate the expression of NDRG2 gene and its role in the progress of salivary adenoid cystic carcinoma (SACC). METHODS: Western blot assay was used to analyze the expression level of protein of SACC. The mRNA level was analyzed by qRT-PCR assay. The expression level of NDRG2 in SACC-LM was downregulated by small interfering RNA. The proliferation of SACC-LM was detected by CCK-8 assay and colony formation assay. Wound healing assay and Transwell invasion assay were performed to observe the abilities of migration and invasion, respectively. The expression of invasion markers and EMT-related proteins was examined by Western blot. SPSS 17.0 software package was used for statistical analysis. RESULTS: NDRG2 expression was decreased in SACC tissues (P=0.017). Down-regulation of NDRG2 promoted proliferation,migration and invasion of SACC-LM (P<0.05). The expression of MMP2 (P=0.0004) was up-regulated. The epithelial marker E-Cadherin was down-regulated (P=0.0229) while the mesenchymal marker N-Cadherin was increased (P=0.0009). CONCLUSIONS: NDRG2 is decreased in SACC and Si-NDRG2 could enhance proliferation,migration and invasion of salivary adenoid cystic carcinoma cell line SACC-LM.

Key words: NDRG2, Salivary adenoid cystic carcinoma, Proliferation, Invasion

CLC Number:

R739.8

REN Hua-jian, LI Jia-su, GAO Hong-mei, YIN Lin. The effect of NDRG2 on proliferation and invasion of salivary adenoid cystic carcinoma[J]. China Journal of Oral and Maxillofacial Surgery, 2019, 17(2): 112-116.

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