Macrophage-derived IL-6 promotes oral cancer cell proliferation

doi:10.19438/j.cjoms.2019.06.004

Abstract

Abstract: PURPOSE: To investigate the effect of macrophage-derived IL-6 on oral cancer cell proliferation. METHODS: The proliferation rates of SCC-15 and CAL-27 cells under different concentration of IL-6 were measured by MTT assay. THP-1 macrophages were induced by PMA, IL-6 mRNA level in LPS-stimulated THP-1 cells were measured by real-time qPCR, IL-6 protein contents in THP-1 conditional medium were measured by ELISA. SCC-15 and CAL-27 cells were co-cultured with THP-1 conditional medium, cell proliferation rate was measured by MTT assay. The data were analyzed by GraphPad 7.0 software package. RESULTS: Recombinant IL-6 in culture medium promoted SCC-15 and CAL-17 cell proliferation; LPS stimulated IL-6 expression in PMA-induced THP-1 macrophages. THP-1 conditional medium promoted SCC-15 and CAL-27 cell proliferation. CONCLUSIONS: IL-6 derived by LPS-stimulated macrophages could promote SCC-15 and CAL-27 cell proliferation.

Key words: Macrophage, Interleukin 6, Oral cancer, Proliferation

CLC Number:

R739.8

LI Jie, CHEN Ju-feng, XIAN Dan. Macrophage-derived IL-6 promotes oral cancer cell proliferation[J]. China Journal of Oral and Maxillofacial Surgery, 2019, 17(6): 503-507.

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