Investigation of circular RNA in oral squamous cell carcinoma

doi:10.19438/j.cjoms.2019.06.003

Abstract

Abstract: PURPOSE: Circular RNA（circRNA） is a special type of non-coding RNA, which is highly conserved and stable. CircRNAs have been reported to be closely associated with the development of various cancers. However, the role of circRNA in oral squamous cell carcinoma (OSCC) remains unclear. This research aimed to investigate the role of circRNAs in OSCC. METHODS: The circRNA expression profiles were obtained by screening 8 pairs of snap-frozen OSCC and paired adjacent normal tissues via high-throughput sequencing. The expression levels of identified circRNAs were further verified in 40 pairs of OSCC tissues by qRT-PCR. Through GraphPad Prism 5.0 analysis, the significantly expressed circRNAs (P<0.05) were selected for further investigation. CircRNA-microRNA (miRNA) network was predicted through various bioinformatics databases. RESULTS: High-throughput sequencing results showed circRNAs were abundantly expressed in OSCC tissues. Hsa_circ_0093229, hsa_circ_0006208 and hsa_circ_0006677 were significantly down-regulated in the cancer tissues. Bioinformatics analysis showed that hsa_circ_0093229, hsa_circ_0006208 and hsa_circ_0006677 might be novel biomarkers of OSCC. CONCLUSIONS: The results revealed that OSCC tissues have abundant circRNAs. Down-regulated hsa_circ_0093229, hsa_circ_0006208 and hsa_circ_0006677 might be novel biomarkers for OSCC.

Key words: Circular RNA, Oral squamous cell carcinoma, Biomarker

CLC Number:

R739.8

WANG Feng, LI Xiang, WANG Yu-fan, YANG Hong-yu. Investigation of circular RNA in oral squamous cell carcinoma[J]. China Journal of Oral and Maxillofacial Surgery, 2019, 17(6): 496-502.

References

[1] Sun ZJ, Chen G, Zhang W, et al.Mammalian target of rapamycin pathway promotes tumor-induced angiogenesis in adenoid cystic carcinoma: its suppression by isoliquiritigenin through dual activation of c-Jun NH2-terminal kinase and inhibition of extracellular signal-regulated kinase[J]. J Pharmacol Exp Therapeut, 2010, 334(2): 500-512.
[2] Wang YF, Zhang W, He KF, et al.Induction of autophagy-dependent cell death by the survivin suppressant YM155 in salivary adenoid cystic carcinoma[J]. Apoptosis, 2014, 19(4): 748-758.
[3] Wang YF, Ma SR, Wang WM, et al.Inhibition of survivin reduces HIF-1alpha, TGF-beta1 and TFE3 in salivary adenoid cystic carcinoma[J]. PloS One, 2014, 9(12): e114051.
[4] Zhang L, Zhang W, Wang YF, et al.Dual induction of apoptotic and autophagic cell death by targeting survivin in head neck squamous cell carcinoma[J]. Cell Death Dis, 2015, 6: e1771.
[5] He KF, Zhang L, Huang CF, et al.CD163+ tumor-associated macrophages correlated with poor prognosis and cancer stem cells in oral squamous cell carcinoma[J]. Biomed Res Int, 2014, 2014: 838632.
[6] Miller KD, Siegel RL, Lin CC, et al.Cancer treatment and survivorship statistics, 2016[J]. CA Cancer J Clin, 2016, 66(4): 271-289.
[7] Qu S, Yang X, Li X, et al.Circular RNA: A new star of noncoding RNAs[J]. Cancer Lett, 2015, 365(2): 141-148.
[8] Jeck WR, Sorrentino JA, Wang K, et al.Circular RNAs are abundant, conserved, and associated with ALU repeats[J]. RNA, 2013, 19(2): 141-157.
[9] Hansen TB, Jensen TI, Clausen BH, et al.Natural RNA circles function as efficient microRNA sponges[J]. Nature, 2013, 495(7441): 384-388.
[10] Memczak S, Jens M, Elefsinioti A, et al.Circular RNAs are a large class of animal RNAs with regulatory potency[J]. Nature, 2013, 495(7441): 333-338.
[11] Yang P, Qiu Z, Jiang Y, et al.Silencing of cZNF292 circular RNA suppresses human glioma tube formation via the Wnt/beta-catenin signaling pathway[J]. Oncotarget, 2016, 7(39): 63449-63455.
[12] Wan L, Zhang L, Fan K, et al.Circular RNA-ITCH suppresses lung cancer proliferation via inhibiting the Wnt/beta-Catenin pathway[J]. Biomed Res Int, 2016, 2016: 1579490.
[13] Li F, Zhang L, Li W, et al.Circular RNA ITCH has inhibitory effect on ESCC by suppressing the Wnt/beta-catenin pathway[J]. Oncotarget, 2015, 6(8): 6001-6013.
[14] Peng L, Chen G, Zhu Z, et al.Circular RNA ZNF609 functions as a competitive endogenous RNA to regulate AKT3 expression by sponging miR-150-5p in Hirschsprung's disease[J]. Oncotarget, 2016, 8(1): 808-818.
[15] Huang SH, O'Sullivan B. Overview of the 8th edition TNM classification for head and neck cancer[J]. Curr Treat Options Oncol, 2017, 18(7): 40.
[16] Glazar P, Papavasileiou P, Rajewsky N. circBase: a database for circular RNAs[J]. RNA, 2014, 20(11): 1666-1670.
[17] Chen LL, Yang L.Regulation of circRNA biogenesis[J]. RNA Biol, 2015, 12(4): 381-388.
[18] Salzman J, Gawad C, Wang PL, et al.Circular RNAs are the predominant transcript isoform from hundreds of human genes in diverse cell types[J]. PloS One, 2012, 7(2): e30733.
[19] Jeck WR, Sharpless NE.Detecting and characterizing circular RNAs[J]. Nat Biotechnol, 2014, 32(5): 453-461.
[20] Conn SJ, Pillman KA, Toubia J, et al.The RNA binding protein quaking regulates formation of circRNAs[J]. Cell, 2015, 160(6): 1125-1134.
[21] Lewinska A, Adamczyk-Grochala J, Kwasniewicz E, et al.Reduced levels of methyltransferase DNMT2 sensitize human fibroblasts to oxidative stress and DNA damage that is accompanied by changes in proliferation-related miRNA expression[J]. Redox Biol, 2018, 14: 20-34.
[22] Duronio RJ, Marzluff WF.Coordinating cell cycle-regulated histone gene expression through assembly and function of the Histone Locus Body[J]. RNA Biol, 2017, 14(6): 726-738.
[23] Luo G, Zhou Y, Yi W, et al.Expression levels of JNK associated with polymorphic lactotransferrin haplotypes in human nasopharyngeal carcinoma[J]. Oncol Lett, 2016, 12(2): 1085-1094.
[24] Islam F, Gopalan V, Law S, et al.MiR-498 in esophageal squamous cell carcinoma: clinicopathological impacts and functional interactions[J]. Hum Pathol, 2017, 62: 141-151.
[25] Dong S, Wang R, Wang H, et al.HOXD-AS1 promotes the epithelial to mesenchymal transition of ovarian cancer cells by regulating miR-186-5p and PIK3R3[J]. J Exp Clin Cancer Res, 2019, 38(1): 110.
[26] Zhao Z, Han C, Liu J, et al.GPC5, a tumor suppressor, is regulated by miR-620 in lung adenocarcinoma[J]. Mol Med Rep, 2014, 9(6): 2540-2546.
[27] Ni XF, Zhao LH, Li G, et al.MicroRNA-548-3p and MicroRNA-576-5p enhance the migration and invasion of esophageal squamous cell carcinoma cells via NRIP1 down-regulation[J]. Neoplasma, 2018, 65(6):881-887.
[28] Sun C, Sang M, Li S, et al.Hsa-miR-139-5p inhibits proliferation and causes apoptosis associated with down-regulation of c-Met[J]. Oncotarget, 2015, 6(37):39756-39792.