Effect of soybean isoflavoneon on osteoclastic differentiation of mandible bone marrow-derived macrophages

doi:10.19438/j.cjoms.2021.03.002

Abstract

Abstract: PURPOSE: To study the effects of soybean isoflavoneon on osteoclastic differentiation of mandible bone marrow-derived macrophages (mBMMs). METHODS: Four-week-old female S-D rats were used to obtain mBMMs. CCK-8 assay was used to analyze the changes of cell viability of mBMMs due to different concentration of soybean isoflavoneon. The effect of soybean isoflavoneon on osteoclastic formation and osteoclastic gene expression was determined by tartrate resistant acid phosphatase staining (TRAP) and q-PCR. SPSS16.0 software package was used for data analysis. RESULTS: Cell viability of mBMMs treated with 1 μmol/L, 100 nmol/L, 10 nmol/L and 1 nmol/L soybean isoflavoneon was comparable with the control group, while100 μmol/L and10 μmol/L soybean isoflavoneon inhibited cell viability of mBMMs (P<0.05). With TRAP staining, both 1 μmol/L and 100 nmol/L soybean isoflavoneon decreased the number osteoclast in BMMs induced by RANKL (P<0.05). Moreover, the mRNA level of NFATc1, MMP9, Trap and Ctsk of in mBMMs treated with 1 μmol/L and 100 nmol/L soybean isoflavoneon decreased significantly in comparison with the control group. CONCLUSIONS: Soybean isoflavoneon inhibits osteoclastic differentiation of mBMMs.

Key words: Soybean isoflavoneon, Mandible bone marrow-derived macrophages, mBMMs, Osteoclastic differentiation, Osteoporosis

CLC Number:

R782.3.2

CHEN Fu-min, DAI Qing-gang, WU Chun-lan, WANG Yue-ping. Effect of soybean isoflavoneon on osteoclastic differentiation of mandible bone marrow-derived macrophages[J]. China Journal of Oral and Maxillofacial Surgery, 2021, 19(3): 197-200.

References

[1] Dai QG, Zhang P, Wu YQ, et al.Ovariectomy induces osteoporosis in the maxillary alveolar bone: an in vivo micro-CT and histomorphometric analysis in rats[J]. Oral Dis, 2014, 20(5): 514-520.
[2] Zhou S, Yang Y, Ha N, et al.The specific morphological features of alveolar bone[J]. J Craniofac Surg, 2018, 29(5): 1216-1219.
[3] Santana N, Mehazabin S, Sangeetha K, et al.Osteodystrophies of jaws[J]. J Oral Maxillofac Pathol, 2020, 24(2): 405-412.
[4] Xu H, Zhou S, Qu R, et al.Icariin prevents oestrogen deficiency-induced alveolar bone loss through promoting osteogenesis via STAT3[J]. Cell Prolif, 2020, 53(2): e12743.
[5] Stewart S, Hanning R.Building osteoporosis prevention into dental practice[J]. J Can Dent Assoc, 2012, 78: c29-c40.
[6] Guiglia R, Di Fede O, Lo Russo L, et al.Osteoporosis, jawbones and periodontal disease[J]. Med Oral Patol Oral Cir Bucal, 2013, 18(1): e93-e99.
[7] Koduganti RR, Gorthi C, Reddy PV, et al.Osteoporosis: "A risk factor for periodontitis"[J]. J Indian Soc Periodontol, 2009, 13(2): 90-96.
[8] Rachner TD, Khosla S, Hofbauer LC.Osteoporosis: now and the future[J]. Lancet, 2011, 377(9773): 1276-1287.
[9] NIH Consensus Development Panel on Osteoporosis Prevention, Diagnosis,Therapy. Osteoporosis prevention, diagnosis, and therapy[J]. JAMA, 2001, 285(6): 785-795.
[10] Brown JP, Josse RG.2002 clinical practice guidelines for the diagnosis and management of osteoporosis in Canada[J]. CMAJ, 2002, 167(10 Suppl): S1-S34.
[11] Zheng X, Lee SK, Chun OK.Soy isoflavones and osteoporotic bone loss: a review with an emphasis on modulation of bone remodeling[J]. J Med Food, 2016, 19(1): 1-14.
[12] Li HF, Zhang P, Tian ZF, et al.Differential mechanisms involved in effects of genistein and 17-beta-estradiol on porcine coronary arteries[J]. Pharmazie, 2006, 61(5): 461-465.
[13] Yamaguchi M, Weitzmann MN.The estrogen 17beta-estradiol and phytoestrogen genistein mediate differential effects on osteoblastic NF-kappaB activity[J]. Int J Mol Med, 2009, 23(2): 297-301.
[14] Liu K, Ma G, Lv G, et al.Effects of soybean isoflavone dosage and exercise on the serum markers of bone metabolism in ovariectomized rats[J]. Asia Pac J Clin Nutr, 2007, 16( Suppl 1): 193-195.
[15] Uesugi T, Fukui Y, Yamori Y.Beneficial effects of soybean isoflavone supplementation on bone metabolism and serum lipids in postmenopausal japanese women: a four-week study[J]. J Am Coll Nutr, 2002, 21(2): 97-102.
[16] 陶若奇, 张建东, 顾九君, 等, 大豆异黄酮对去势大鼠骨质疏松性骨折愈合的影响[J]. 临床骨科杂志, 2015, 18(1): 110-113.
[17] Sliwinski L, Folwarczna J, Janiec W, et al.Differential effects of genistein, estradiol and raloxifene on rat osteoclasts in vitro[J]. Pharmacol Rep, 2005, 57(3): 352-359.
[18] Katz J, Rotstein I.Prevalence of periapical lesions in patients with osteoporosis[J]. J Endod, 2021, 47(2): 234-238.
[19] Amaro ERS, Ortiz FR, Dorneles LS, et al. Estrogen protects dental roots from orthodontic-induced inflammatory resorption[J]. Arch Oral Biol, 2020, 117: 104820.e1-e8.
[20] Durie BGM, Katz M, Crowley J, et al.Osteonecrosis of the jaw and bisphosphonates[J]. N Engl J Med, 2005, 353(1): 99-102.
[21] Chien HI, Chen LW, Liu WC, et al.Bisphosphonate-related osteonecrosis of the jaw[J]. Ann Plast Surg, 2021, 86(2S Suppl 1): S78-S83.
[22] Ryu JI, Kim HY, Kwon YD.Is implant surgery a risk factor for osteonecrosis of the jaw in older adult patients with osteoporosis? A national cohort propensity score-matched study[J]. Clin Oral Implants Res, 2021, 32(4): 437-447.
[23] Yu F, Liu Z, Tong Z, et al.Soybean isoflavone treatment induces osteoblast differentiation and proliferation by regulating analysis of Wnt/beta-catenin pathway[J]. Gene, 2015, 573(2): 273-277.
[24] Lu M, Xie K, Huang K, et al.Effects of soybean isoflavone on metabolism of rat osteoblasts and cytokines in vitro[J]. J Food Sci, 2020, 85(4): 1302-1306.