The effect of apoptosis and epithelial-mesenchymal transition during palatal fusion

doi:10.19438/j.cjoms.2018.06.003

Abstract

Abstract: PURPOSE: Disappearance mechanism of the medial edge epithelium (MES) in the process of palatal fusion is still unknown. The aim of this study was to investigate the effect of apoptosis and epithelial-mesenchymal transition (EMT) during palatal fusion. METHODS: 8-week-old C57BL/6J inbred mice were selected as the experimental mouse. The palatal shelves were acquired at E13.5, E14.0, E14.5, E15.5 and E17.5. Immunohistochemistry and TUNEL were used to explore the protein expression and cell apoptosis during the key process of palatal fusion. Three pictures were randomly selected at each time point, and gray images were analyzed by Image J software. SPSS12.0 software package was used for statistical analysis. RESULTS: Immunohistochemistry and TUNEL assay found that the expressions of fibronectin and vimentin in MES and apoptosis were detected during the critical stage of palate fusion (E14.5), and the two processes were detected in the anterior, middle and posterior palate shelves. CONCLUSIONS: EMT and apoptosis were involved in the degeneration of MES, and the fusion mechanism of the anterior palate was the same as the posterior palate.

Key words: Cleft palate, Palatal shelves, Apoptosis, Epithelial-mesenchymal transition

CLC Number:

R782.2

MA Li, SHI Bing, ZHANG Dong-sheng, ZHENG Qian. The effect of apoptosis and epithelial-mesenchymal transition during palatal fusion[J]. China Journal of Oral and Maxillofacial Surgery, 2018, 16(6): 493-497.

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