Study on Twist1 promoting epithelial-mesenchymal transition and drug-resistance in tongue squamous cell carcinoma

doi:10.19438/j.cjoms.2018.01.001

Abstract

Abstract: PURPOSE: The aim of this study was to investigate the mechanism of Twist1 regulating epithelial-mesenchymal transition (EMT) and cisplatin-resistance in tongue squamous carcinoma cells. METHODS: TSCC CDDP resistant cell line CAL27/CDDP was transfected with Twist1 siRNA. Western blot and immunofluorescence staining were used to detect the expression of E-cadherin and Vimentin; Western blot was used to detect the expression of Twist1 and Slug; Transwell assay and scratch test were used to detect the invasion and migration capabilities; MTT was used to analyze the half maximal inhibitory concentration (IC₅₀) values. One-way ANOVA was used to analyze the results with SPSS 17.0 software package. RESULTS: Twist1 siRNA transfection reversed EMT phenotype of CAL27/CDDP. The expression of E-cadherin was up-regulated and the expression of Vimentin, Twist1 and Slug was down-regulated. The motility was significantly decreased. The IC₅₀ of CAL27/CDDP decreased by 41.75%±5.10%（P<0.01). CONCLUSIONS: Twist1 promotes EMT and CDDP drug-resistance in TSCC.

Key words: Tongue squamous cell carcinoma, Epithelial-mesenchymal transition, Twist1, Drug resistance

CLC Number:

R739.8

LIU Mo, RUAN Yi, LI Jing-song, HUANG Hong-zhang. Study on Twist1 promoting epithelial-mesenchymal transition and drug-resistance in tongue squamous cell carcinoma[J]. China Journal of Oral and Maxillofacial Surgery, 2018, 16(1): 1-5.

References

[1] Pang MF, Georgoudaki AM, Lambut L, et al.TGF-β1-induced EMT promotes targeted migration of breast cancer cells through the lymphatic system by the activation of CCR7/CCL21-mediated chemotaxis[J]. Oncogene, 2016, 35(6): 748-760.
[2] Zhu DJ, Chen XW, Zhang WJ, et al.Twist1 is a potential prognostic marker for colorectal cancer and associated with chemoresistance[J]. Am J Cancer Res, 2015, 5(6): 2000-2011.
[3] Qin Y, Zhang Q, Lee S, et al.Doxycycline reverses epithelial-to-mesenchymal transition and suppresses the proliferation and metastasis of lung cancer cells[J]. Oncotarget, 2015, 6(38): 40667-40679.
[4] Li CW, Xia W, Lim SO, et al.AKT1 inhibits epithelial-to-mesenchymal transition in breast cancer through phosphorylation-dependent Twist1degradation[J]. Cancer Res, 2016, 76(6): 1451-1462.
[5] Wushou A, Pan HY, Liu W, et al.Correlation of increased Twist1 with lymph node metastasis in patients with oral squamous cell carcinoma[J]. J Oral Maxillofac Surg, 2012, 70(6): 1473-1479.
[6] 刘墨, 张斌, 王安训, 等. 人舌鳞状细胞癌顺铂耐药细胞发生上皮-间质转化的研究[J].中华口腔医学研究杂志, 2014, 8(5): 5-9.
[7] 刘墨, 张斌, 王成, 等. 转化生长因子 β 1 诱导人舌鳞状细胞癌细胞上皮-间质转化及其顺铂耐药性研究[J]. 中华口腔医学研究杂志, 2012, 6(3): 223-231.
[8] Sun L, Yao Y, Liu B, et al.MiR-200b and miR-15b regulate chemotherapy-induced epithelial-mesenchymal transition in human tongue cancer cells by targeting BMI1[J]. Oncogene, 2012, 31(4): 432-445.
[9] Cheng GZ, Chan J, Wang Q, et al.Twist1 transcriptionally up-regulates AKT2 in breast cancer cells leading to increased migration, invasion, and resistance to paclitaxel[J]. Cancer Res, 2007, 67(5): 1979-1987.
[10] Saxena M, Stephens MA, Pathak H, et al.Transcription factors that mediate epithelial-mesenchymal transition lead to multidrug resistance by upregulating ABC transporters[J]. Cell Death Dis, 2011, 2(7): e179.
[11] Leptin M.Twist1 and snail as positive and negative regulators during Drosophila mesoderm development[J]. Genes Dev, 1991, 5(6): 1568-1576.
[12] Casas E, Kim J, Bendesky A, et al.Snail2 is an essential mediator of Twist1-induced epithelial-mesenchymal transition and metastasis[J]. Cancer Res, 2011, 71(1): 245-254.
[13] van Nes JG, de Kruijf EM, Putter H, et al. Co-expression of SNAIL and TWIST1 determines prognosis in estrogen receptor-positive early breast cancer patients[J]. Breast Cancer Res Treat, 2012, 133(1): 49-59.
[14] Yang MH, Chen CL, Chau GY, et al.Comprehensive analysis of the independent effect of Twist1 and snail in promoting metastasis of hepatocellular carcinoma[J]. Hepatology, 2009, 50(5): 1464-1474.
[15] Jouppila-Mättö A, Närkiö-Mäkelä M, Soini Y, et al.Twist1 and snai1 expression in pharyngeal squamous cell carcinoma stroma is related to cancer progression[J].BMC Cancer, 2011, 11: 350.