Study on immunogenic cell death induced by thermo-chemotherapy in CAL-27 cells of tongue squamous cell carcinoma

doi:10.19438/j.cjoms.2020.02.004

Abstract

Abstract: PURPOSE： To investigate whether pingyangmycin(PYM), hyperthermia(HT) and their combination could induce immunogenic cell death of CAL-27 cells. METHODS: CCK-8 assay, flow cytometry and ELISA assay were used to detect the effect of different treatments on CAL-27 cell proliferation, apoptosis, membrane expression rate of CRT and HMGB1 secretion. SPSS 20.0 software package was used for statistical analysis of the data. RESULTS: PYM inhibited CAL-27 cell activity in a concentration-dependent manner (P<0.05). The membrane expression rate of CRT after PYM treatment and HT treatment were significantly higher than that in the untreated group. After treatment of PYM combined with HT, apoptosis, CRT membrane expression rate and HMGB1 secretion were all increased significantly compared with untreated group, chemotherapy group and HT group (P<0.05). CONCLUSIONS: PYM chemotherapy and HT could induce CRT translocation from intracellular to membrane surface of CAL-27 cells, and combined application of PYM and HT was significantly better than that of PYM alone in inducing apoptosis and immunogenic cell death.

Key words: Tongue, Squamous cell carcinoma, Hyperthermia, Thermochemo-therapy, Immunogenicity, Calreticulin, HMGB1

CLC Number:

R739.8

SUN Qiao-zhen, SHI Fan, LUO Dan, XU Ting, WANG Sheng-zhi. Study on immunogenic cell death induced by thermo-chemotherapy in CAL-27 cells of tongue squamous cell carcinoma[J]. China Journal of Oral and Maxillofacial Surgery, 2020, 18(2): 111-116.

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