The influence of maxillary sinus outer wall osteotomy on maxillary sinus mucosa function

Abstract

Abstract: PURPOSE: To investigate the expression of MUC5AC in maxillary sinus mucosa of rabbits after maxillary sinus outer wall osteotomy, and explore the influence of outer wall osteotomy on maxillary sinus mucosa function. METHODS: Twelve healthy adult New Zealand white rabbits receiving outer wall osteotomy were selected to establish surgical models. Maxillary vestibular groove mucosa was incised after general anesthesia. A 20 mm×2 mm bone and mucosa defect was established on the outer wall of the left maxillary sinus, which was used as experimental side. Right maxillary sinus was untreated as negative control group. All experimental animals were randomized into 3 groups and sacrificed 2, 4 and 8 weeks after surgery, respectively. The morphologic change of maxillary sinus mucosa and secretion of goblet cells were examined through H-E staining and immunohistochemistry. The data were analyzed by using Graphpad Prism v5.0 software package. RESULTS: Two weeks after surgery, cilium fell off from maxillary sinus mucosa, glands destroyed and decreased in number. Mucosa cells were swollen like balloons with glands proliferation 4 week after surgery. Epithelium returned to nearly normal morphology, while the proportion of cilium cells was decreased, with irregular arrangement and abnormal morphology. Immunohistochemistry showed expression level of MUC5AC in the experimental group was significantly higher than in the control group (P<0.001). Expression level was strongest in the 2-week group and slightly declined in the 4-week group, while weakest in the 8-week group. CONCLUSIONS: Maxillary sinus mucous secretion changed significantly after outer wall osteotomy. Tissue structures could repair partially over time, but are still different from normal structures. Minimizing sinus mucosal damage during surgery may reduce the impact on its physiological functions.

Key words: Rabbit, Maxillary sinus outer wall osteotomy, Maxillary sinus mucosa, MUC5AC

CLC Number:

R782.2

LIU Xiao-yu, BAI Xi-jing, YANG Xue-wen. The influence of maxillary sinus outer wall osteotomy on maxillary sinus mucosa function[J]. China Journal of Oral and Maxillofacial Surgery, 2016, 14(6): 500-504.

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