Abstract: PURPOSE: To investigate the application value of cervical lymphatic imaging enhanced by submucosal injection of C/Gd nanocomposite as a new lymphoid contrast agent in interstitial magnetic resonance lymphography (IMRLG). METHODS: 0.2 mL C/Gd nanocomposite was injected into the bilateral tongue submucosa in 12 healthy New Zealand white rabbits and then massaged the injection site for 30 s. MR images were obtained before injection and 5,10,15,20 and 40 mins after injection by using 3D enhancement magnetic resonance lymphography. The signal intensities were measured to calculate the enhancing rates (E%) and the signal enhancing rates-time curve was drawn before and after enhancing of cervical lymph nodes. The data were statistically analyzed by using SPSS17.0 software package. RESULTS: After injection, C/Gd nanocomposite was rapidly absorbed into the cervical drainage area. The lymph nodes and lymphatic vessels were strengthened significantly; however, the blood vessels were not displayed at the same time. The enhancing rates of the cervical lymph nodes reached a peak 10 mins after injection, and the best enhancing effect was between 5 mins and 20 mins. It would not be able to distinguish from that of the surrounding tissues. CONCLUSIONS: As an IMRLG contrast agent, C/Gd nanocomposite can characteristically display the cervical lymph node morphology and lymphatic shape effectively with a small dose.

Key words: Cervical lymph node, Nano-carbon, Interstitial lymphography, Gadolinium, New Zealand white rabbit