Assessment of cervical lymph nodes metastasis using interstitial MR lymphography with C/Gd nanocomposite in a rabbit tongue cancer model

doi:10.19438/j.cjoms.2019.02.002

Abstract

Abstract: PURPOSE: To investigate the value of a novel imaging agent C/Gd nanocomposite interstitial magnetic resonance lymphography (IMRLG) in the diagnosis of cervical sentinel lymph node metastasis in a rabbit model of VX2 tongue cancer. METHODS: VX2 cancer tissue blocks were implanted into the left ventral submucosa of tongues in 16 healthy New Zealand white rabbits. After 25 days of implanting VX2, 0.2 mL of contrast agent C/Gd nanocomposite were injected into bilateral submucosa of rabbits on both sides. MR lymphography was performed at 5 min, 10 min, 15 min, 20 min, 30 min, 40 min and 24 h after injection. The signal enhancement rate (E%) was calculated, the sentinel lymph nodes was located and the characteristics of the lymphatic system was observed. After 24 hours of injecting contrast agent, 0.2 mL of methylene blue were injected into the bilateral submucosa for lymph staining. Subsequently, the cervical lymph nodes were dissected and histopathological examinations were performed. SPSS19.0 software package was used to analyze the data. RESULTS: Sixteen rabbit models with tongue transplanted tumor were successfully established. The cervical lymph node metastases were found in 16 rabbits. E% had significant difference between the tumor side and the control side ten minutes after injection of the contrast agent (P<0.05). IMRLG showed 18 metastatic nodes. Pathological examination indicated that 17/18 were positive, yielding a success rate of 94.45% (17/18). There was no significant difference between the two methods for diagnosing SLN metastasis (P>0.05). CONCLUSIONS: IMRLG by C/Gd nanocomplexes can be used to accurately determine the status of sentinel lymph node, which effectively provides a new way for detecting metastasis of lymph node of of tongue carcinoma.

Key words: Tongue cancer, Nano-carbon, MR, Interstitial lymphography, Sentinel lymph node

CLC Number:

R782

XU Hao-yue, LIU Shi-en, ZHU Hai-tao, SONG Kai, FENG Yuan-yong, WU Da-xiong, SHANG Wei. Assessment of cervical lymph nodes metastasis using interstitial MR lymphography with C/Gd nanocomposite in a rabbit tongue cancer model[J]. China Journal of Oral and Maxillofacial Surgery, 2019, 17(2): 106-111.

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