Osteogenesis ability of bone marrow-derived mesenchymal stem cells derived from diabetic rabbits

doi:10.19438/j.cjoms.2017.02.002

Abstract

Abstract: PURPOSE : To observe the biological characteristics (mainly osteogenesis ability) of bone marrow-derived mesenchymal stem cells (BMSCs) in diabetic rabbits. METHODS : Diabetic rabbit models were established by injecting alloxan intravenously, which were used as the experimental group, rabbits receiving normal saline injection were served as the control group. Rabbit iliac bone marrow was extracted under aseptics and local anesthesia, and BMSCs were isolated and cultured by using whole blood adherent culture method. MTT assay, real-time PCR, and alkaline phosphatase (ALP) staining of the BMSCs in both groups were performed, respectively. Differences between groups were analyzed with SPSS 13.0 software package for ANOVA and SNK post hoc analysis. RESULTS : Compared with the control group, the ability of cell proliferation, osteogenic gene expression, ALP activity and angiogenic gene expression of the diabetic rabbits BMSCs were all decreased, and the difference was statistically significant (P<0.05). CONCLUSION S: The activity of cell proliferation, osteogenesis and angiogenesis of the diabetic rabbit BMSCs is damaged.

Key words: Diabetic rabbit, BMSCs, Proliferation activity, Osteogenesis, Angiogenesis

CLC Number:

R329.2

ZHOU Yu-ning, MA Wu-di, GUI Zhi-peng, XIA Lun-guo, XU Yuan-jin. Osteogenesis ability of bone marrow-derived mesenchymal stem cells derived from diabetic rabbits[J]. China Journal of Oral and Maxillofacial Surgery, 2017, 15(2): 103-108.

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