Effect of sympathetic denervation on alternation of NE/adrb3 expression and MSCs distribution in rat distraction osteogenesis model

Abstract

Abstract: PURPOSE: To determine the alternation of NE/adrb3, Nestin expression in rat model after sympathetic deneration during distraction osteogenesis. METHODS: According to different intervention and time point, 20 adult male SD rats were divided into 4 groups (n=5) equally. Rats in the experimental group underwent right mandible distraction osteogenesis and transection of cervical sympathetic trunk (TCST), while rats in the control group only underwent right mandible distraction osteogenesis. The right mandibles and the distraction zone specimens were harvested for immunohistochemistry assays of NE/ adrb3 expression and mesenchymal stem cells (MSCs) distribution at specific time point. The data were analyzed using SPSS 13.0 software package. RESULTS: The expression of NE was hardly found, and very weak adrb3 expression was observed around the blood vessel in TCST group, but the expression of NE and adrb3 was abundant in the control group; the Nestin+ MSCs were abundant and mainly distributed in callus matrix in TCST group, whereas that Nestin+ MSCs were mainly located adjacent to perivascular region in the control group. CONCLUSIONS: TCST depleted sympathetic mediator NE and its receptor adrb3 in distraction zone; Nestin+ MSCs have higher tendency of migration from perivascular region to callus matrix under distraction stress after TCST in vivo.

Key words: Distraction osteogenesis, Transection of cervical sympathetic trunk, Norepinephrine, Beta 3 adrenergic receptor, Mesenchymal stem cells

CLC Number:

DU Zhao-jie, ZHANG Ya-bo, FU Tao, SHI Dong-yue, YANG Qing-tian. Effect of sympathetic denervation on alternation of NE/adrb3 expression and MSCs distribution in rat distraction osteogenesis model[J]. China Journal of Oral and Maxillofacial Surgery, 2016, 14(6): 505-509.

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