China Journal of Oral and Maxillofacial Surgery ›› 2022, Vol. 20 ›› Issue (4): 334-339.doi: 10.19438/j.cjoms.2022.04.004

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Cu-bearing titanium alloy based on three-dimensional printing promoted osteogenic differentiation of bone marrow mesenchymal stem cells

JIANG Sheng-jie, MEI Peng, ZHOU Hao-nan, PAN Zhao-cheng, GAO Yuan, CAI Ming   

  1. Department of Oral and Craniomaxillofacial Surgery, Shanghai Ninth People's Hospital, Shanghai Jiao Tong University School of Medicine; College of Stomatology, Shanghai Jiao Tong University; National Center for Stomatology; National Clinical Research Center for Oral Diseases; Shanghai Key Laboratory of Stomatology. Shanghai 200011, China
  • Received:2022-03-03 Revised:2022-04-17 Online:2022-07-20 Published:2022-07-20

Abstract: PURPOSE: To explore the biocompatibility and the properties to enhance osteogenic differentiation of bone marrow mesenchymal stem cells (BMSCs) by Cu-bearing titanium alloy based on three-dimensional printing. METHODS: Cu-bearing titanium alloy based on three-dimensional printing(3D Ti-5Cu) was fabricated using Ti-6Al-4V-5Cu alloy powders as raw material via selective laser melting(SLM) technology. The 3D Ti-5Cu alloy was co-cultured with BMSCs to observe its effect on cell activity, adhesion, proliferation, alkaline phosphatase(ALP) activity and gene expression related to osteogenic differentiation, medical titanium alloy (Ti-6Al-4V) was set as the control group. The data were analyzed with GraphPad Prism 9.3.1 software. RESULTS: 3D Ti-5Cu alloy with good cytocompatibility was successfully fabricated in this study, and no statistical difference was found compared with the control group(P>0.05). Meanwhile, 3D Ti-5Cu alloy could enhance adhesion and proliferation of BMSCs, and significantly increased ALP activity and the expression levels of osteogenic differentiation genes such as Runx2, Alp, Bmp2, Opn and Col1a1, and significant differences were found between the groups(P<0.05). CONCLUSIONS: 3D Ti-5Cu alloy possesses excellent biocompatibility and can further promote the osteogenic differentiation of BMSCs, which is beneficial to improve the biological inertia of titanium alloys, and provides a theoretical basis for the realization of biological functionalization of titanium alloys.

Key words: Three-dimensional printing, Cu-bearing titanium alloy, Bone marrow mesenchymal stem cells, Osteogenic differentiation

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