Effect of condylar bone marrow-derived mesenchymal stem cells on chondrogenic differentiation in young rats induced by nasal obstruction

doi:10.19438/j.cjoms.2017.01.001

Abstract

Abstract: PURPOSE: This study was designed to utilize the animal model of nasal obstruction to investigate its effect on chondrogenic differentiation of condylar bone marrow-derived mesenchymal stem cells (BMSCs). METHODS: Forty 4-week-old SD rats were randomly divided into the bilateral nasal obstruction group (28 rats) and the control group (12 rats). The animal experiment lasted for 4 weeks, from 8:00 to 16:00 every day. During modeling, bilateral condylar BMSCs were cultured in vitro, CCK-8 was used to compare the difference of cell proliferation,real-time polymerase chain reaction (RT-PCR) was used to detect the difference of gene expression of BMSCs chondrogenic marker genes (Agg, COL-Ⅱ, SOX -9). The data were statistically analyzed using SPSS19.0 software package. RESULTS: There was no significant difference of condylar BMSCs proliferation ability between the two groups (P>0.05), but the expression of chondrogenic marker genes were all significantly lower in the experimental group (P<0.05). CONCLUSIONS: It is suggested that the mechanism of mandibular deficiency caused by long-term mouth breathing is closely related to the inhibition of condylar BMSCs to cartilage differentiation.

Key words: Nasal obstruction, Mandible, Condyle, Bone marrow -derived mesenchymal stem cells, Chondrogenesis

CLC Number:

Q254

WANG Xiao-ling, SUN Hui-jun, ZHU Yan-fei, TANG Yan-mei, XUE Xiao-chen, NIE Ping, ZHU Min. Effect of condylar bone marrow-derived mesenchymal stem cells on chondrogenic differentiation in young rats induced by nasal obstruction[J]. China Journal of Oral and Maxillofacial Surgery, 2017, 15(1): 1-5.

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