Macroporous calcium phosphate cement, human embryonic stem cells and platelet-rich plasma constructs for bone regeneration in cranial defects in rats

doi:10.19438/j.cjoms.2017.01.002

Abstract

Abstract: PURPOSE: To investigate the effects of macroporous calcium phosphate cement (CPC), human embryonic stem cells (hESC) and platelet-rich plasma constructs for bone regeneration in vivo in critical-sized cranial defects in rats. METHODS: Three groups were tested in rats: (a) CPC with hESC-derived mesenchymal stem cells (CPC+hESC-MSCs); (b) CPC with hESC-MSCs and 30% platelet rich plasma (PRP) (CPC+hESC-MSCs+30%PRP) and (c) CPC+hESC-MSCs+50%PRP. 18 rats were sacrificed 12 weeks after operation. Micro-CT and bone histomorphologic analysis were conducted to compare the status of new bone formation. SPSS16.0 software package was used for one way analysis of variance. RESULTS: At 12 weeks in vivo, the groups with hESC-MSCs and PRP had more new bone formation compared with other two groups; however, there was no significant difference in new bone area fraction between different concentration of PRP groups. CONCLUSIONS: Macroporous CPC with hESC-MSCs and PRP may be useful for bone regeneration in craniofacial and orthopedic fields.

Key words: Calcium phosphate cement, Human embryonic stem cells, Platelet rich plasma, Bone tissue engineering

CLC Number:

R782.2

WU Zhen-zhen, BAO Chong-yun, LI Ming-zheng, LIU Xian. Macroporous calcium phosphate cement, human embryonic stem cells and platelet-rich plasma constructs for bone regeneration in cranial defects in rats[J]. China Journal of Oral and Maxillofacial Surgery, 2017, 15(1): 6-10.

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