Preparation and property of digital drug-loaded chlorhexidine gluconate PLA/nHA composite scaffold

doi:10.19438/j.cjoms.2022.01.003

Abstract

Abstract: PURPOSE: To investigate the physical and chemical properties, cytocompatibility and antibacterial activity of 3D printed porous polylactic acid/nano-hydroxyapatite(PLA/nHA) scaffolds loaded with chlorhexidine gluconate chitosan nanoparticles (CSn-CG). METHODS: By means of ion crosslinking, CSn (blank chitosan nanospheres) and CSn-CG were prepared. The morphology of the nanospheres was observed under transmission electron microscopy(TEM). PLA/nHA scaffolds were prepared using PLA and nHA as raw materials by digital design and 3D printing technology, and the scaffolds were loaded with CSn and CSn-CG by immersion method. The experiment was divided into PLA/nHA group, PLA/nHA/CSn group and PLA/nHA/CSn-CG group. The scaffolds were characterized by scanning electron microscopy (SEM), X-ray photoelectron spectroscopy (XPS), Fourier transform infrared spectroscopy (FTIR) and X-ray diffraction(XRD). In vitro release test was used to evaluate the sustained-release performance of the scaffolds. CCK-8 method was used to evaluate the biocompatibility of scaffolds. The antibacterial effect of the stent was detected by AGAR diffusion method. SPSS 25.0 software package was used for data analysis. RESULTS: CSn-CG was homogeneous nanospheres. SEM showed that the scaffolds in each group were all three-dimensional network structure with regular pore size. The results of in vitro release showed that CG could be released slowly from the stent at low speed, and the time lasted for 30 days. CCK-8 results showed that PLA/nHA/CSn-CG promoted proliferation of MC3T3-E1. In vitro antibacterial experiment results showed that the experimental group had obvious antibacterial circle, which indicated that the scaffold had good antibacterial effect. CONCLUSIONS: The 3D-printed PLA/nHA/CSn-CG scaffold has good physical and chemical property, biocompatibility and antibacterial effect in vitro, which provides basis for further application of tissue engineered bone.

Key words: Chitosan, Chlorhexidine, 3D printing, Composite scaffolds, Bone tissue engineering

CLC Number:

ZHANG Xiu-xiu, SUN Jian, LI Ya-li, LIU Yan-shan, CHEN Li-qiang, XU Yao-xiang, XU Ze-xian, MENG Kun, SUN Ming, ZHOU Dong-yang, XU Dian, GAO Xiao-han, CHENG Li-di. Preparation and property of digital drug-loaded chlorhexidine gluconate PLA/nHA composite scaffold[J]. China Journal of Oral and Maxillofacial Surgery, 2022, 20(1): 15-21.

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