China Journal of Oral and Maxillofacial Surgery ›› 2014, Vol. 12 ›› Issue (3): 193-197.

• Basic Research Articles •     Next Articles

Serum peptide analysis in oral squamous cell carcinoma

LIU Ying, TAN Yi-ran, YANG Cheng-zhe, ZHU Dong-wang, ZHONG Lai-ping, ZHANG Chen-ping, ZHANG Zhi-yuan   

  1. Department of Oromaxillofacial Head and Neck Oncology, Ninth People’s Hospital, College of Stomatology, Shanghai Jiao Tong University School of Medicine; Shanghai Key Laboratory of Stomatology. Shanghai 200011, China
  • Received:2013-09-16 Online:2014-05-10 Published:2014-07-15

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Abstract: PURPOSE: To explore serum peptide profiles in patients with oral squamous cell carcinoma (OSCC) at early and late clinical stages. METHODS: Matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS) combined with weak cation magnetic nano-beads (WCX) were used to detect serum peptide profiles in 120 healthy persons, 37 OSCC patients at early clinical stage and 129 OSCC patients at late clinical stage. ClinprotTools 2.1TM software was used to analyze different serum peptide peaks and establish differential diagnosis model. Potential significant peaks were identified by searching IPI database. RESULTS: A total of 385 differential serum peptide peaks were found among healthy persons, early stage OSCC patients and late stage OSCC patients. Based on the differential diagnosis model, five peptide peaks were identified as FGA, ITIH4, PKM2, SCARA5 and SDPR, which could separate OSCC patients at early clinical stage from healthy person with sensitivity of 82.4% and specificity of 98.5%; four peptide peaks were identified as FGA, PKM2, ITIH4 and ALB, which could separate OSCC patients at early clinical stage from those at late clinical stage with sensitivity of 98.5% and specificity of 88.2%; five peptide peaks could be used to separate OSCC patients at late clinical stage from healthy persons with sensitivity of 89.4% and specificity of 85.1%, three of them were identified as SERPINA1,TTR and SDPR. CONCLUSION: From these results, potential differential serum peptides are identified to separate OSCC patients from healthy persons. These potential serum biomarkers should be further validated with mechanism studies before application in clinic. Supported by National Natural Science Foundation of China (81272979), Research Fund of Science and Technology Committee of Shanghai Municipality (1052nm04700 and 13QH1401700), and Medicine and Engineering Cross Research Fund of Shanghai Jiao Tong University (YG2010MS49).

Key words: Oral squamous cell carcinoma, Matrix-assisted laser desorption time of flight mass, Magnetic nano-beads, Serum peptide

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