Single-cell transcription atlas for mandibular condyle cartilage and subchondral bone marrow in rat temporomandibular joint osteoarthritis

doi:10.19438/j.cjoms.2023.04.003

Abstract

Abstract: PURPOSE: To establish the single-cell transcriptome atlas for rat condyle osteochondral tissue and bone marrow both in normal and temporomandibular joint osteoarthritis (TMJOA) condition. METHODS: Rat unilateral anterior crossbite(UAC) model was built to induce TMJOA. Osteochondral cells and bone marrow immune cells were extracted for single-cell RNA sequencing. Quality control, dimension reducing, clustering and marker gene detection were used to depict cell subclusters. Differential gene expression analysis and functional enrichment were utilized to demonstrate cell cluster functions and their changes under UAC. Pseudo-time analysis was applied for cell development trajectory, while cell communication prediction was used to show alternations in the interaction among cell clusters. RESULTS: There were 9 cell clusters in rat condyle, containing osteochondral cells and bone marrow immune cells. Under UAC, the number of osteochondrocytes decreased whereas immune cells accumulated. Condyle cartilage and bone tissue contained 6 functional groups of cells. Among them, D and F group maintained progenitor characteristics and possessed the ability to develop into other groups. In UAC condition, osteochondrocytes witnessed impaired extracellular matrix organization, activated senescent or inflammatory pathways, and diminished cell-cell interaction. Meanwhile, in the bone marrow, neutrophils showed enhanced chemotaxis and degranulation, mononuclear-phagocytes displayed increased phagosomes, activated B cells were also recruited. CONCLUSIONS: There are progenitor cells in condyle cartilage and bone tissue, whose function impaired under UAC. At the same time, immune cells in the bone marrow are activated. Targeted modulation of osteochondral progenitors and immune cells could contribute in alleviation the condyle tissue damage in TMJOA.

Key words: Temporomandibular joint osteoarthritis, Malocclusion, Single-cell transcriptome, Progenitor cells, Immune cells, Rat

CLC Number:

R782.6

YU Ye-ke, DING Ruo-yi, SUN Jia-li, ZHANG Zhi-yuan, HE Dong-mei. Single-cell transcription atlas for mandibular condyle cartilage and subchondral bone marrow in rat temporomandibular joint osteoarthritis[J]. China Journal of Oral and Maxillofacial Surgery, 2023, 21(4): 332-339.

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