The expression of beta adrenergic receptor in CAL-27 cell line of tongue carcinoma and the effects of beta blockers on proliferation, migration and invasion

doi:10.19438/j.cjoms.2018.05.002

Abstract

Abstract: PURPOSE: To investigate the expression of β adrenergic receptors in CAL-27 cell line of tongue carcinoma and the effects of β-blockers on the proliferation , migration and invasion of CAL-27 cells. METHODS: Normal oral mucosal epithelial cells (NOMEC) were cultured and identified. Immunohistochemistry and real-time quantitative PCR(qRT-PCR) were applied to detect the expression of β-adrenergic receptor in CAL-27 and oral mucosal epithelial cells. The proliferation, migration and invasion of CAL-27 cells were investigated by CCK-8 method, scratch adhesion test and Transwell assay under different concentrations of propranolol and metoprolol. Experimental data were analyzed with analysis of variance and t test by SPSS 16.0 software package. RESULTS: The expression of β1-AR and β2-AR in CAL-27 cells were higher than that in NOMEC, while the expression of β2-AR at mRNA level were lower than that in NOMEC, the expression of β1-AR was higher than that in NOMEC. CCK-8 assay showed with the increase of drug concentration, the inhibitory effect on CAL-27 cell proliferation was enhanced. When the drug concentration was >50 μmol/L, the inhibiting effect of propranolol on cell proliferation was significant (P<0.05). Scratch-wound assay showed that when drug concentration was >2.5 μmol/L, migration of CAL-27 cells was inhibited (P<0.05), and propranolol had a greater inhibitive effect. Transwell assay showed that when drug concentration >1.0 μmol/L,invasion of CAL-27 cells was suppressed (P<0.05), the suppression effect of propranolol was greater. CONCLUSIONS: The expression of β1-AR in CAL-27 cells is significantly higher than that in NOMEC and the expression of β2-AR is lower than that in NOMEC. Low concentration of propranolol and metoprolol has no significant effect on proliferation of CAL-27 cells, but can inhibit the migration and invasion of CAL-27 cells.

Key words: β-AR, CAL-27 cells, Metoprolol, Propranolol

CLC Number:

R739.8

LIU Shi-qi, YANG Liang-hui, CHEN Guo-sheng, WANG hui, FANG fang, LIAO Xian-xiang, MAI Hua-ming. The expression of beta adrenergic receptor in CAL-27 cell line of tongue carcinoma and the effects of beta blockers on proliferation, migration and invasion[J]. China Journal of Oral and Maxillofacial Surgery, 2018, 16(5): 391-396.

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