Effects and mechanism of quercetin on proliferation and apoptosis of human adenoid cystic carcinoma cell line ACC-M

doi:10.19438/j.cjoms.2017.02.005

Abstract

Abstract: PURPOSE : To observe the effect of quercetin on human adenoid cystic carcinoma cells (ACC-M) proliferation and apoptosis. METHODS : CCK-8 staining was used to detect different concentrations of quercetin on ACC-M cell proliferation inhibition, the inhibition rate and IC₅₀ were calculated; flow cytometry was used to detect ACC-M cell apoptosis rate and cell cycle distribution; Survivin mRNA of ACC-M cells was detected by RT-PCR. The difference between each group was analyzed with SPSS20.0 software package. RESULTS : Quercetin inhibited ACC-M cell growth obviously in a concentration dependent manner. The IC₅₀ was 151.12 μmol/L (P<0.05); annexin V/PI double staining assay showed that quercetin can induce apoptosis of ACC-M cells in a dose and time dependent manner (P<0.05); PI staining assay showed that quercetin played an important role in G2/M cells, which were specifically arrested in G2/M phase. RT-PCR showed the quercetin concentration was negatively related with survivin mRNA in ACC-M cells and 200 μmol/L quercetin could inhibit the expression of Survivin mRNA significantly. CONCLUSION S: Quercetin can inhibit the proliferation of ACC-M cells and induce its apoptosis in a time and dose dependent manner by down-regulating the level of Survivin.

Key words: Quercetin, Adenoid cystic carcinoma, Cell proliferation, Cell apoptosis, Survivin

CLC Number:

R739.8

MI Jing, ZHANG Ming-bin, WAN Guang-yong. Effects and mechanism of quercetin on proliferation and apoptosis of human adenoid cystic carcinoma cell line ACC-M[J]. China Journal of Oral and Maxillofacial Surgery, 2017, 15(2): 121-126.

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