Effect of interferon inducible protein-10 on proliferation and apoptosis of tongue squamous cancer cell

doi:10.19438/j.cjoms.2018.01.004

Abstract

Abstract: PURPOSE: To investigate the influence of interferon inducible protein-10 (IP-10) on proliferation, apoptosis of tongue squamous cancer cell CAL-27 , and the relation with CXCR3A, CXCR3B gene expression. METHODS: CAL-27 cells were divided into 3 experimental group and 1 control group. The experimental groups were treated with 10 ng/mL, 20 ng/mL, and 40 ng/mL IP-10, respectively; while the control group was not stimulated. CCK 8 was used to test cell proliferation after being stimulated for 12, 24, 48, and 72 h; Flow cytometry was used to detect cell apoptosis after being stimulated for 24 h. Q-PCR was used to detect CXCR3A, CXCR3B mRNA expression after being stimulated for 12, 24, 48 and 72 h, respectively. SPSS 16.0 software package was used for statistical analysis. RESULTS: IP-10 with 3 kinds of concentration promoted CAL-27 cell proliferation (P<0.05) at 12, 24 h. At 48h, only 40 ng/mL of IP-10 promoted CAL-27 cell proliferation (P<0.01). At 72 h, IP-10 with 3 kinds of concentration had no effect on CAL-27 cell proliferation (P>0.05). After 24 h, CAL 27 cell proliferation in 3 experimental groups decreased compared with the control group. At 24 h, cell apoptosis rate in 3 experimental groups was 3.377%±0.575%, 6.867%±2.848%, 5.317%±2.794%, respectively. There was significant difference between the control group (P<0.05), but no significant difference between the experimental groups (P>0.05). At 12, 24, 48, 72 h, CXCR3A mRNA expression was 0.0130±0.0007, 0.0274±0.0005, 0.0204±0.0011 and 0.0174±0.0006 respectively; there was significant difference between groups (P<0.05). CXCR3B mRNA expression was 0.0124±0.0015, 0.0209±0.0016, 0.0297±0.0013 and 0.0386±0.0010, respectively; there was significant difference between groups (P<0.05). CONCLUSIONS: IP-10 can not only promote CAL-27 proliferation, but also induce its apoptosis. As time goes on, cell proliferation decreased, CXCR3A mRNA expression increased first and then decreased, while CXCR3B mRNA expression increased gradually. CXCR3A, CXCR3B may participate in regulation of tongue squamous cancer cell proliferation and apoptosis induced by IP-10.

Key words: Tongue squamous cell carcinoma, Tongue squamous cell carcinoma cell lines, CXC-chemokine receptor 3, Interferon inducible protein-10, Cell proliferation, Cell apoptosis

CLC Number:

R739.8

CHU Hong-xing, JIA Bo, ZHANG Zhao-qiang, WANG Zhi-ping, CHEN Jun, ZHENG Xiang-huai, SUN Xiang, ZHAO Jian-jiang. Effect of interferon inducible protein-10 on proliferation and apoptosis of tongue squamous cancer cell[J]. China Journal of Oral and Maxillofacial Surgery, 2018, 16(1): 20-24.

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