Expression of Smad7 protein in oral squamous cell carcinoma and the impact on cell proliferation and migration of oral squamous cell carcinoma

doi:10.19438/j.cjoms.2017.03.006

Abstract

Abstract: PURPOSE: To investigate the expression of Smad7 in oral squamous cell carcinoma (OSCC) and determine if Smad7 was associated with tumor cell proliferation and migration. METHODS: Thirty-one primary OSCC patients were enrolled in this study. Smad7 expression in tissue specimens were investigated by using immunohistochemistry staining, CCK-8, cell scratch assay, western blotting and qRT- PCR were performed after Smad7 siRNA interference in HN4 cells. Paired t test was used for analysis with SPSS18.0 software package. RESULTS: Nuclear and cytoplasmic expression of Smad7 was increased in primary OSCC tissue samples. Expression of Smad7 was associated with cervical lymph node metastasis. Smad7 knockdown significantly suppressed cell proliferation but promoted tumor cell migration in HN4 cells. Smad7 knockdown promoted N-cadherin and Vimentin expression. CONCLUSIONS: Smad7 expression in OSCC was associated with cervical lymph nodal metastasis and Smad7 might play an essential role in the proliferation and migration of OSCC cells.

Key words: Smad7 protein, Oral squamous cell carcinoma, Western blot, RNA interference

CLC Number:

R783.3

ZHENG Chen, SHI Chao-ji, ZHENG Bo, JIN Xiao-ming, JIANG Yin-hua.. Expression of Smad7 protein in oral squamous cell carcinoma and the impact on cell proliferation and migration of oral squamous cell carcinoma[J]. China Journal of Oral and Maxillofacial Surgery, 2017, 15(3): 220-226.

References

[1] Hunter KD, Parkinson EK, Harrison PR. Profiling early head and neck cancer [J]. Nat Rev Cancer, 2005, (2): 127-135.
[2] Forastiere A, Koch W, Trotti A, et al. Head and neck cancer [J]. N Engl J Med, 2001, 26(345): 1890-1900.
[3] Hayashida T, Jinno H, Kitagawa Y, et al. Cooperation of cancer stem cell properties and epithelial-mesenchymal transition in the establishment of breast cancer metastasis [J]. J Oncol, 2011, 2011: 591427.
[4] Micalizzi DS, Farabaugh SM, Ford HL. Epithelial-Mesenchymal transition in cancer: parallels between normal development and tumor progression [J]. J Mammary Gland Biol Neoplasia, 2010, 15(2): 117-134.
[5] 孙青, 江培洲, 沈新明, 等. 肿瘤转移的分子机制及其调控因素研究进展 [J]. 国际肿瘤学杂志, 2000, 27(4): 216-218.
[6] Yang J, Mani SA, Donaher JL, et al. Twist, a master regulator of morphogenesis, plays an essential role in tumor metastasis [J]. Cell, 2004, 117(7): 927-939.
[7] Zhang Y, Yu Z, Xiao Q, et al. Expression of BAMBI and its combination with Smad7 correlates with tumor invasion and poor prognosis in gastric cancer[J]. Tumor Biol, 2014, 35(7): 7047-7056.
[8] Kim SY, Chu KC, Lee HR, et al. Establishment and characterization of nine new head and neck cancer cell lines [J]. Acta Otolaryngol, 1997, 117(5): 775-784.
[9] Hata A, Shi Y, MAssague J. TGF-beta signaling and cancer: structural and functional consequences of mutations in Smads [J]. Mol Med Today, 1998, 4(6): 257-262.
[10] 霍艳英. TGF-β及Smads信号在癌症中的作用[J]. 国外医学·肿瘤学分册, 2002, 29(3): 163-165.
[11] Kleeff J, Ishiwata T, Maruyama H, et al. The TGF-beta signaling inhibitor Smad7 enhances tumorigenicity in pancreatic cancer [J]. Oncogene, 1999, 18(39): 5363-5372.
[12] Boulay JL, Mild G, Lowy A, et al. SMAD7 is a prognostic marker in patients with colorectal cancer [J]. Int J Cancer, 2003, 104(4): 446-449.
[13] Ginos MA, Page GP, Michalowicz BS, et al. Identification of a gene expression signature associated with recurrent disease in squamous cell carcinoma of the head and neck [J]. Cancer Res,

[1]	REZIWANGULI·Yasen, MAIREPATI·Maiming, LI Chen-xi, GONG Zhong-cheng. Research progress in the role and mechanism of Porphyromonas gingivalis in promoting oral squamous cell carcinoma [J]. China Journal of Oral and Maxillofacial Surgery, 2023, 21(2): 186-190.
[2]	LAN Rong, RUAN Min, HAN Nan-nan, WANG Feng. Peri-implant squamous cell carcinoma appeared after implant-restoration in the left mandible: a case report [J]. China Journal of Oral and Maxillofacial Surgery, 2023, 21(2): 203-205.
[3]	LIU Heng, DUAN Xiao-feng. Research progress of ferritinophagy and related genes FTH1 and NCOA4 in oral squamous cell carcinoma [J]. China Journal of Oral and Maxillofacial Surgery, 2023, 21(1): 87-91.
[4]	JI Ping-ping, LI Li, WU Yi-fan, HOU Li-li. Effect of transcutaneous electrical acupoint stimulation on myelosuppression in patients with oral squamous cell carcinoma after induction chemotherapy [J]. China Journal of Oral and Maxillofacial Surgery, 2022, 20(5): 455-460.
[5]	XU Gang, SONG Qi-yi, JIANG Wei-dong, LIU Jing-xiu. Expression and clinical significance of aquaporin 3 in oral mucosal epithelial dysplasia and oral squamous cell carcinoma [J]. China Journal of Oral and Maxillofacial Surgery, 2022, 20(5): 478-482.
[6]	YANG Song, WANG Ning, ZHAO Shu-chao, LI Heng-yong, GE Sheng-you, ZHOU Kai, SONG Kai, SHANG Wei, FENG Yuan-yong. Expression and clinical significance of chemerin in oral squamous cell carcinoma patients combined with type 2 diabetes [J]. China Journal of Oral and Maxillofacial Surgery, 2022, 20(4): 390-395.
[7]	ZHANG Yi-yi, YANG Yue-yi, JU Wu-tong, ZHONG Lai-ping. Neoadjuvant immunotarget therapy in patients with locally advanced oral squamous cell carcinoma: report of 2 cases and literature review [J]. China Journal of Oral and Maxillofacial Surgery, 2022, 20(4): 413-416.
[8]	WEI Dong-liang, LI Zhi, JU Hou-yu, WU Yun-teng, GUO Wei, REN Guo-xin. Mechanism of EGFR-targeted drugs for pain relief in oral cancer [J]. China Journal of Oral and Maxillofacial Surgery, 2022, 20(3): 219-224.
[9]	SUN Da-wei, ZHANG Yun, ZHU Yong. Effect of shRNA interference Rce1gene expression on the radiosensitivity of oral squamous cell carcinoma cells [J]. China Journal of Oral and Maxillofacial Surgery, 2022, 20(2): 123-128.
[10]	NUERMANGULI·Yasen, DUOLIKUN·Wufuer, BAIERNA·Wushuoer, YAN Guang-peng. The relationship between serum IL-6, IL-17, TNF-α, IFN-γ levels, clinical characteristics of60patients with oral squamous cell carcinoma [J]. China Journal of Oral and Maxillofacial Surgery, 2022, 20(2): 141-145.
[11]	QU Chu-xiang, SUN Shu-yang, ZHANG Zhi-yuan. Understanding oral cancer invasion from the perspective of genetic heterogeneity [J]. China Journal of Oral and Maxillofacial Surgery, 2022, 20(2): 193-197.
[12]	WANG Yu-xin, WANG Zhi-yong, WANG Yong-gong, WANG Hui-ming, WANG Li-zhen, TIAN Hao, YE Jin-hai, FU Kun, RUAN Min, SUN Chang-fu, SUN Guo-wen, LIU Fa-yu, XU Bi-yun, HOU Jun, LIU Bing, LIU Liang, CHEN Yong-feng, CHEN Zhan-wei, XIAO Can, LI Si-yi, LI Zhi-yong, LI Yong, LI Nan, ZHANG Chen-ping, ZHANG Dong-sheng, ZHANG Sheng, ZHANG Kai, YANG Xi, HE Wei, SU Tong, JI Tong, LIN Li-song, LUO Rui-hua, ZHAO Zhi-li, HU Qin-gang, TANG Zhan-gui, XU Wei-jia, HUANG Zhi-quan, HUANG Xiao-feng, LIANG Yu-jie, HAN Zheng-xue, HAN Wei, JIANG Can-hua, YU Jian-jun, CAI Zhi-gang, LIAO Gui-qing, LIAO Sheng-kai, CAI Hui-ming, WEI Jian-hua. Chinese expert consensus statement on the application of of indocyanine green(ICG) fluorescence imaging in the treatment of oral squamous cell carcinoma [J]. China Journal of Oral and Maxillofacial Surgery, 2022, 20(1): 1-6.
[13]	LI Xing, XU Fei-hu, YANG Yun-bo, HAN Nan-nan, RUAN Min. Clinicopathological characteristics and prognostic analysis of oral squamous cell carcinoma with parotid lymph node metastasis [J]. China Journal of Oral and Maxillofacial Surgery, 2022, 20(1): 28-31.
[14]	YANG Yun-bo, WANG Yu-pu, LI Hang, HAN Nan-nan, YAN Ming, RUAN Min. Expression and clinical significance of cystatin A in oral squamous cell carcinoma [J]. China Journal of Oral and Maxillofacial Surgery, 2021, 19(6): 494-499.
[15]	HUANG Jin-yun, ZHANG Xin-yi, JU Hou-yu, JI Hong-hai, REN Guo-xin. Correlation between oral microbial homeostasis and cisplatin sensitivity in patients with oral squamous cell carcinoma [J]. China Journal of Oral and Maxillofacial Surgery, 2021, 19(6): 500-505.