The growth-promoting function of HN12 cell on neurites under the condition of coculture with trigeminal ganglion of SD rat

Abstract

Abstract: PURPOSE: To build a new in vitro study model of neoneurogenesis, to study the influence of HN12 cell on trigeminal ganglion of SD rat under the condition of coculture. METHODS: Mouse trigeminal ganglion (TG) and human oral squamous cancer cells (HN12) were co-cultured in Matrigel matrix. Control cultures consisted of oral squamous cancer cells alone and TG alone. Neurite outgrowth was quantitated with dark phase microscopy and image analysis(Optimas 6.1) at 24,48,72,96 and 120 h. SPSS 19.0 software package was used for statistical analysis. RESULTS: HN-12 cell had a strong influence on the neurites growth compared with control group after 5 days of consecutive observation. CONCLUSION: HN-12/TG coculture model was successfully built. HN-12 cell has a strong influence on the neurites growth.

Key words: Oral squamous cell carcinoma, Trigeminal ganglion, Co-culture, Neoneurogenesis

CLC Number:

R739.8

LU Wei, YU Zhi-wei, ZHANG Jian-jun, WANG Li-zhen, JI Tong.. The growth-promoting function of HN12 cell on neurites under the condition of coculture with trigeminal ganglion of SD rat[J]. China Journal of Oral and Maxillofacial Surgery, 2014, 12(4): 307-310.

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