Co-culture of rat bone marrow mesenchymal stem cells and condylar chondrocytes induces chondrogenic differentiation

Abstract

Abstract: PURPOSE: To investigate the influence of co-culture of rat bone marrow mesenchymal stem cells (BMSCs) and condylar chondrocytes on chondrogenic differentiation of BMSCs. METHODS: BMSCs and condylar chondrocytes were isolated and cultured from SD rat. Passage 2 BMSCs and chondrocytes (1.2×10⁴/mL) were co-cultured at a ratio of 2 to 1 as co-culture group, condylar chondrocytes with same concentration and passage as experimental group, BMSCs with same concentration and passage as control group . Inverted microscope was used to observe cell morphology; CCK-8 was used to evaluate the proliferation of the cells; alcian blue staining, alkaline phosphatase(ALP) staining and quantitative analysis were used to evaluate cell differentiation; Western blot was used to detect the expression of COL2A1,COL10A1. One-way ANOVA with SPSS13.0 software package was performed for statistical analysis. RESULTS: The cells of co-culture group and experimental group were polygon-shaped and displayed cobble-stone morphology which were typical morphology of chondrocytes, while cells in the control group were long spindle-shaped; cell growth curve indicated cells in the co-culture group had greater proliferation ability compared with the other two groups; alcian blue and ALP staining of cells in the co-culture group and experimental group were positive ;the expression of COL2A1 and COL10A1 were significantly increased. CONCLUSIONS: BMSCs can maintain the phenotype of condylar chondrocytes and promote proliferation of chondrocytes. At the same time, condylar chondrocytes can induce BMSCs to chondrogenic differentiation.

Key words: Condyle, Chondrocytes, Bone marrow mesenchymal stem cells, Co-culture, Seed cell

CLC Number:

Q254

SHENG Xia-han, QU Lu-yao, CAI Xie-yi. Co-culture of rat bone marrow mesenchymal stem cells and condylar chondrocytes induces chondrogenic differentiation[J]. China Journal of Oral and Maxillofacial Surgery, 2016, 14(6): 490-494.

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