In vitro study of the radiosensitivity of bone marrow stem cells isolated from Sprague-Dawley rats

China Journal of Oral and Maxillofacial Surgery ›› 2015, Vol. 13 ›› Issue (3): 205-210.

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In vitro study of the radiosensitivity of bone marrow stem cells isolated from Sprague-Dawley rats

LIU Zhong-long¹, DAI Tian-guo¹, FU Shui-ting¹, HU Hai-sheng², HE Yue¹

1.Department of Oromaxillofacial Head and Neck Oncology, Shanghai 200011, China;
2.Department of Radiotherapy, Shanghai Ninth People’s Hospital, College of Stomatology, Shanghai Jiao Tong University School of Medicine; Shanghai Key Laboratory of Stomatology. Shanghai 200011, China

Received:2014-10-14 Revised:2015-01-24 Online:2015-05-20 Published:2015-06-18
Supported by:
Supported by National Natural Science Foundation of China (81271112), Development Foundation of Shanghai Municipal Human Resources and Social Security Bureau (201312), and SMC Rising Star (A) Scholar of Shanghai Jiao Tong University

Abstract

Abstract: PURPOSE: To investigate the radiosensitivity of bone marrow stem cells (BMSCs) isolated from mice in vitro. METHODS: BMSCs was isolated from Sprague-Dawley rats and subsequently cultured in vitro. Third passage of BMSCs was exposed under ionizing radiation with various doses (0,4,8,10 Gy). Cell proliferation was detected using CCK-8 method and flow cytometry was applied to identify the cell apoptosis. Real-time PCR was used to measure the gene expression of Cyclin-D1 and Casepase-3 at 24 hour and 7 day. The difference between various treatment groups was analyzed with SPSS 20.0 software package. RESULTS: BMSCs were successfully isolated and cultured in vitro. Cell growth curve analysis indicated that BMSCs with 10 Gy treatment showed faster growth compared with the control group. Meanwhile, group with 10 Gy irradiation showed higher proportion of died cells and apoptotic cells than those treated with other doses. Significant up-regulation of Cyclin-D1 was found in group with 10 Gy irradiation at 24 hour (P<0.01) and 7 day (P<0.001).Up-regulation of Casepase-3 was only found at 24 hour (P<0.01). CONCLUSIONS: BMSCs of SD rats showed radioresistance following ionizing radiation in vitro.

Key words: Bone marrow stem cells, Ionizing radiation, Cell proliferation, Cell apoptosis

CLC Number:

Q274

LIU Zhong-long, DAI Tian-guo, FU Shui-ting, HU Hai-sheng, HE Yue. In vitro study of the radiosensitivity of bone marrow stem cells isolated from Sprague-Dawley rats[J]. China Journal of Oral and Maxillofacial Surgery, 2015, 13(3): 205-210.

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In vitro study of the radiosensitivity of bone marrow stem cells isolated from Sprague-Dawley rats

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