热疗对Cal27细胞Id-1、HSF1表达的影响

doi:10.19438/j.cjoms.2018.02.009

中国口腔颌面外科杂志 ›› 2018, Vol. 16 ›› Issue (2): 144-148.doi: 10.19438/j.cjoms.2018.02.009

热疗对Cal27细胞Id-1、HSF1表达的影响

孙俊伟, 罗丹, 刘浩, 王升志

青岛大学附属烟台毓璜顶医院口腔颌面外科,山东烟台 264000;

收稿日期:2017-09-14 修回日期:2017-11-18 出版日期:2018-03-20 发布日期:2018-04-08
通讯作者: 王升志,E-mail：wangsz916@163.com
作者简介:孙俊伟（1990-）,男,在读硕士研究生,E-mail：754459692@qq.com
基金资助:
山东省科技发展计划项目（2014GSF118184）

Effect of hyperthermia on Id-1,HSF1 expression in Cal27 cell line

SUN Jun-wei, LUO Dan, LIU Hao, WANG Sheng-zhi

Department of Oral and Maxillofacial Surgery, The Affiliated Yantai Yuhuangding Hospital of Qingdao University. Yantai 264000, Shandong Province, China;

Received:2017-09-14 Revised:2017-11-18 Online:2018-03-20 Published:2018-04-08

摘要/Abstract

摘要： 目的: 探讨热疗对人舌癌Cal27细胞中Id-1、HSF1基因表达水平的影响,以及对Cal27细胞增殖、迁徙的影响。 方法: 采用实时荧光定量PCR检测43℃、1 h热疗对Cal27细胞Id-1、HSF1基因表达的影响;CCK-8实验和划痕实验检测Cal27细胞增殖活性、迁徙能力的变化。应用SPSS19.0软件包对数据进行统计学分析。 结果: 热疗后Id-1的mRNA表达水平降低,HSF1的mRNA表达水平升高;热疗后2 h,实验组Cal27细胞增殖活性显著降低（P<0.05）;热疗可以显著抑制Cal27细胞的迁徙能力（P<0.05）。 结论: 热疗可抑制Cal27细胞的增殖活性、迁徙能力,降低Id-1的表达,升高HSF1的表达。

关键词: 热疗, Id-1, HSF1, 舌鳞状细胞癌, Cal27

Abstract: PURPOSE: To investigate the influence of hyperthermia on expression of Id-1, HSF1 in Cal27 cell and the effect on Cal27 cell proliferation and migration. METHODS: Cal27 cell lines were hyperthermia-treated (43℃ for 1 h); 4 h later, real-time quantitative transcriptase-polymerase chain reaction was used to detect the expressions of Id-1, HSF1. Cell proliferation and migration were observed by CCK-8 and wound healing. SPSS 19.0 software package was used to analyze the data. RESULTS: The mRNA level of Id-1 was decreased and the mRNA level of HSF1 was increased after hyperthermia. The proliferation of Cal27 cell descended at 2 h post-hyperthermia. Migration of Cal27 cell descended after hyperthermia treatment. The difference was statistically significant (P<0.05). CONCLUSIONS: Hyperthermia can inhibit Cal27 cell proliferation and migration ability, reduce the mRNA level of Id-1 and increase the mRNA level of HSF1.

Key words: Hyperthermia, Id-1, HSF1, Tongue squamous cell carcinoma, Cal27

中图分类号:

R739.8

孙俊伟, 罗丹, 刘浩, 王升志. 热疗对Cal27细胞Id-1、HSF1表达的影响[J]. 中国口腔颌面外科杂志, 2018, 16(2): 144-148.

SUN Jun-wei, LUO Dan, LIU Hao, WANG Sheng-zhi. Effect of hyperthermia on Id-1,HSF1 expression in Cal27 cell line[J]. China J Oral Maxillofac Surg, 2018, 16(2): 144-148.

参考文献

[1] Gao S, Zheng M, Ren X, et al.Local hyperthermia in head and neck cancer: mechanism, application and advance[J]. Oncotarget, 2016, 7(35): 57367-57378.
[2] Benezra R, Davis RL, Lockshon D, et al.The protein Id: a negative regulator of helix-loop-helix DNA binding proteins[J]. Cell, 1990, 61(1): 49-59.
[3] Zhao J, Wang S, Liu N, et al.Correlation between the expression of Id-1 and hyperthermia-associated molecules in oral squamous cell carcinoma[J]. J Clin Pathol, 2013, 66(9): 758-763.
[4] Wazir U, Jiang WG, Sharma AK, et al.The mRNA expression of inhibitors of DNA binding-1 and -2 is associated with advanced tumour stage and adverse clinical outcome in human breast cancer[J]. Anticancer Res, 2013, 33(5): 2179-2183.
[5] Ponz-Sarvisé M, Castanoñ E, Panizo-Santos A, et al.Differential tumor expression of inhibitor of differentiation-1 in prostate cancer patients with extreme clinical phenotypes and prognostic implications[J]. Clin Genitourin Cancer, 2014, 12(2): 87-93.
[6] Kubelac MP, Fetica B, Vlad IC, et al.The role of inhibitor of DNA-binding 1 (Id--1) protein and angiogenesis in serous ovarian cancer[J]. Anticancer Res, 2014, 34(1): 413-416.
[7] Zhou JD, Yang L, Zhu XW, et al.Clinical significance of up-regulated Id-1 expression in Chinese de novo acute myeloid leukemia[J]. Int J Clin Exp Pathol, 2015, 8(5): 5336-5344.
[8] Iliakis G, Krieg T, Guan J, et al.Evidence for an S-phase checkpoint regulating DNA replication after heat shock: a review[J]. Int J Hyperthermia, 2004, 20(2): 240-249.
[9] Yunoki T, Kariya A, Kondo T, et al.The combination of silencing BAG3 and inhibition of the JNK pathway enhances hyperthermia sensitivity in human oral squamous cell carcinoma cells[J]. Cancer Lett, 2013, 335(1): 52-57.
[10] Toraya-Brown S, Sheen MR, Zhang P, et al.Local hyperthermia treatment of tumors induces CD8(+) T cell-mediated resistance against distal and secondary tumors[J]. Nanomedicine, 2014, 10(6): 1273-1285.
[11] Dayanc BE, Bansal S, Gure AO, et al.Enhanced sensitivity of colon tumour cells to natural killer cell cytotoxicity after mild thermal stress is regulated through HSF1-mediated expression of MICA[J]. Int J Hyperthermia, 2013, 29(5): 480-490.
[12] Richter K, Haslbeck M, Buchner J.The heat shock response: life on the verge of death[J]. Mol Cell, 2010, 40(2): 253-266.
[13] Akerfelt M, Morimoto RI, Sistonen L.Heat shock factors: integrators of cell stress, development and lifespan[J]. Nat Rev Mol Cell Biol, 2010, 11(8): 545-555.
[14] Kim HY, Kim YS, Yun HH, et al.ERK-mediated phosphorylation of BIS regulates nuclear translocation of HSF1 under oxidative stress[J]. Exp Mol Med, 2016, 48(9): e260.
[15] Shah SP, Nooka AK, Jaye DL, et al.Bortezomib-induced heat shock response protects multiple myeloma cells and is activated by heat shock factor 1 serine 326 phosphorylation[J]. Oncotarget, 2016, 7(37): 59727-59741.
[16] Lasorella A, Benezra R, Iavarone A.The ID proteins: master regulators of cancer stem cells and tumour aggressiveness[J]. Nat Rev Cancer, 2014, 14(2): 77-91.
[17] Chen KY, Chen CC, Tseng YL, et al.GCIP functions as a tumor suppressor in non-small cell lung cancer by suppressing Id-1-mediated tumor promotion[J]. Oncotarget, 2014, 5(13): 5017-5028.
[18] Shin DH, Park JH, Lee JY, et al.Overexpression of Id-1 in transgenic mice promotes mammary basal stem cell activity and breast tumorigenesis[J]. Oncotarget, 2015, 6(19): 17276-17290.
[19] 武雪亮, 王立坤, 杨东东, 等. siRNA干扰Id--1表达对人结肠癌SW480细胞株生长与侵袭能力影响研究[J]. 中华肿瘤防治杂志, 2017, 24(3): 156-160.
[20] Gumireddy K, Li A, Kossenkov AV, et al.Id-1 promotes breast cancer metastasis by S100A9 regulation[J]. Mol Cancer Res, 2014, 12(9): 1334-1343.
[21] Ma H, Wei Y, Leng Y, et al.TGF-beta1-induced expression of Id-1 is associated with tumor progression in gastric cancer[J]. Med Oncol, 2014, 31(7): 19.
[22] Jin H, Xie X, Hu B, et al.Hyperthermia inhibits the proliferation and invasive ability of mouse malignant melanoma through TGF-β₍₁)[J]. Oncol Rep, 2013, 29(2): 725-734.
[23] Wang L, Man N, Sun XJ, et al.Regulation of AKT signaling by Id-1 controls t(8;21) leukemia initiation and progression[J]. Blood, 2015, 126(5): 640-650.
[24] Man J, Shoemake JD, Ma T, et al.Hyperthermia sensitizes glioma stem-like cells to radiation by inhibiting AKT signaling[J]. Cancer Res, 2015, 75(8): 1760-1769.
[25] Jiang S, Tu K, Fu Q, et al.Multifaceted roles of HSF1 in cancer[J]. Tumour Biol, 2015, 36(7): 4923-4931.

热疗对Cal27细胞Id-1、HSF1表达的影响

Effect of hyperthermia on Id-1,HSF1 expression in Cal27 cell line

PDF (PC)

可视化

摘要/Abstract

引用本文

使用本文

参考文献

相关文章 15

编辑推荐

Metrics

本文评价

[1]	水文军, 高金星, 任蕾. 基质金属蛋白酶9抑制剂对人舌鳞癌SCC-25细胞侵袭、迁移能力的影响及机制探讨[J]. 中国口腔颌面外科杂志, 2021, 19(5): 424-428.
[2]	陈正岗, 王奇民, 童磊, 王云英, 徐晓娜, 王莹, 韩红钰, 盛善桂, 王少如. 沉默Icmt基因对舌鳞癌CAL-27和SCC-4细胞增殖、凋亡及细胞周期的影响[J]. 中国口腔颌面外科杂志, 2021, 19(2): 97-104.
[3]	孔宪琛, 姜晓东, 芦昕, 禹雯怡, 薛娇, 张鹏, 赵璐, 袁荣涛. 核糖体蛋白L29在舌鳞状细胞癌中的表达及意义[J]. 中国口腔颌面外科杂志, 2021, 19(2): 110-115.
[4]	王子昱, 丁宇洁, 陈冰, 沈昊翀, 孟箭, 仝伟凤. α桐酸对舌鳞状细胞癌CAL-27细胞的体外抑制作用[J]. 中国口腔颌面外科杂志, 2020, 18(6): 490-494.
[5]	周光明, 梁衍灿, 黄子贤, 张彬. 长链非编码RNA FOXD2-AS1对舌鳞癌细胞迁移、侵袭的影响[J]. 中国口腔颌面外科杂志, 2020, 18(6): 495-500.
[6]	孙巧珍, 石凡, 罗丹, 徐婷, 王升志. 热化疗诱导舌鳞癌CAL-27细胞免疫原性死亡的实验研究[J]. 中国口腔颌面外科杂志, 2020, 18(2): 111-116.
[7]	陈永菊, 陈伟良, 周斌, 钟江龙, 汪延, 陈睿. 面-颏下动脉岛状皮瓣修复年轻与老年舌癌患者的效果比较[J]. 中国口腔颌面外科杂志, 2020, 18(2): 117-121.
[8]	林志立, 梁建平, 周斌. lncRNA-HOTTIP在低氧诱导舌鳞癌细胞上皮-间质转化过程中的作用探讨[J]. 中国口腔颌面外科杂志, 2019, 17(1): 26-31.
[9]	罗丹, 孙巧珍, 刘少华, 孙俊伟, 王升志. 热疗联合Id-1基因沉默对舌鳞癌CAL-27细胞增殖及侵袭的影响[J]. 中国口腔颌面外科杂志, 2018, 16(6): 488-492.
[10]	刘墨, 李劲松, 阮毅, 黄洪章. miR-181a抑制舌鳞癌细胞上皮-间充质转化及化疗耐药机制的研究[J]. 中国口腔颌面外科杂志, 2018, 16(4): 289-295.
[11]	刘墨, 阮毅, 李劲松, 黄洪章. Twist1调控上皮-间质转化促进舌鳞癌细胞顺铂耐药机制的研究[J]. 中国口腔颌面外科杂志, 2018, 16(1): 1-5.
[12]	褚洪星, 贾搏, 张兆强, 王治平, 陈军, 郑相淮, 孙翔, 赵建江. 干扰素诱导蛋白10对舌鳞癌细胞增殖、凋亡的影响[J]. 中国口腔颌面外科杂志, 2018, 16(1): 20-24.
[13]	吴泽键, 周斌, 陈伟生, 吴晓平, 黄志权. 低氧激活HIF-1α诱导舌鳞癌细胞上皮-间质转化[J]. 中国口腔颌面外科杂志, 2017, 15(2): 136-141.
[14]	周斌,陈伟良,王友元,林钊宇,张大明,范松. 癌相关成纤维细胞对人舌鳞状细胞癌上皮-间质转化的影响[J]. 中国口腔颌面外科杂志, 2014, 12(3): 198-204.
[15]	陈小华;;张斌;;刘墨;王成;刘习强;. 化疗耐药相关蛋白在舌鳞状细胞癌中的表达和意义 [J]. 中国口腔颌面外科杂志, 2013, 11(5): 388-394.