Effects of Icarisid Ⅱ on proliferation and osteogenic differentiation of alveolar bone-derived stem cells in Beagle dogs

doi:10.19438/j.cjoms.2019.04.004

Abstract

Abstract: PURPOSE: To investigate the effect of Icarisid Ⅱon proliferation and osteogenic differentiation of alveolar bone-derived stem cells(AMSCs) in Beagle dogs. Methods: Alveolar bone-derived stem cells were isolated and cultured from the jaw bone of Beagle dogs in vitro. After dAMSCs were stimulated by ICSII at 10^-5, 10^-6, 10^-7, 10^-8 and 10^-9 mol/L, the proliferation and alkaline phosphatase (ACP) activity of dAMSCs were detected by CCK-8 and ALP kit at 1, 3, 5 and 7 days. ALP staining, Alizarin red staining, Western blot and RT-PCR were performed at different time points to evaluate the osteogenic capacity of cells. SPSS22.0 software package was used for statistical analysis. Results: Primary dAMSCs adhered to the wall, showing spindle shape and multidirectional differentiation ability. ICSII can promote the proliferation of dAMSCs at different concentrations. The results showed that ALP activity was also increased, and the formation of calcium nodules was observed at the experimental concentration. The expression levels of each osteogenic gene increased, which were significantly higher than that of the complete medium group. With the extension of time, runx-2 protein expression decreased in the experimental group, OCN protein expression gradually increased, and the gap between the experimental group and the control group widened. OSX protein expression was higher than that of the control group, but there was no significant difference. Conclusions: ICSII can promote the proliferation and osteogenic differentiation of dAMSCs.

Key words: Icarisid Ⅱ, Alveolar bone-derived stem cells, Beagle dogs, Proliferation, Osteogenic differentiation

CLC Number:

R782.2

ZHANG Yi-wen, LUO Yuan, GUO Hua-yan, MIAO Guo-jun, ZHANG Ying-di, HUANG Yuan-liang. Effects of Icarisid Ⅱ on proliferation and osteogenic differentiation of alveolar bone-derived stem cells in Beagle dogs[J]. China Journal of Oral and Maxillofacial Surgery, 2019, 17(4): 304-310.

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