Preliminary screening and validation of cyclin D1 co-expressed long non-coding RNAs in human pleomorphic adenoma

doi:10.19438/j.cjoms.2019.03.002

Abstract

Abstract: PURPOSE: To screen out and validate the differentially expressed long non-coding RNAs (LncRNAs) that co-expressed with cyclin D1 in human pleomorphic adenoma. METHODS: The expression of cyclin D1 in human pleomorphic adenoma was first validated through real time-PCR, Western blot and micro-array analysis. The differentially expressed LncRNAs from the micro-array results that co-expressed with cyclin D1 were screened out via calculating the Pearson's correlation coefficient (PCC), and then the expression of three randomly selected lncRNAs (GSE61474_TCONS_00180431, ENST00000603829 and T253381) were validated with real time PCR. Statistical analysis was completed with SPSS 19.0 software package. RESULTS: The expression of cyclin D1 was significantly up-regulated in human pleomorphic adenoma than the controls (P<0.05). A total of 9 LncRNAs that co-expressed with cyclin D1 were screened out, among which, 6 were up-regulated and 3 were down-regulated. Real-time PCR results demonstrated that same expression trends of the three selected LncRNAs were shown with micro-array results. However, only ENST00000603829 expressed significantly in the subsequent enlarged tumor samples (P<0.05). CONCLUSIONS: The differentially expressed LncRNAs that co-expressed with cyclin D1 were screened out and validated in human pleomorphic adenoma. These LncRNAs could play important roles in pathogenesis and development of pleomorphic adenoma, possibly through interacting with cyclin D1.

Key words: Pleomorphic adenoma, Cyclin D1, Long non-coding RNA

CLC Number:

R739.8

WEI Jun-shui, LU Hao, YANG Wen-jun, SHEN Shu-kun, XU Wan-lin. Preliminary screening and validation of cyclin D1 co-expressed long non-coding RNAs in human pleomorphic adenoma[J]. China Journal of Oral and Maxillofacial Surgery, 2019, 17(3): 198-203.

References

[1] Tian Z, Li L, Wang L, et al.Salivary gland neoplasms in oral and maxillofacial regions: a 23-year retrospective study of 6982 cases in an eastern Chinese population[J]. Int J Oral Maxillofac Surg, 2010, 39(3): 235-242.
[2] Eveson JW PA, Gnepp DR, El-Naggar AK.Tumours of the salivary glands [M]. 3rd Ed. Lyon: IARC Press, 2005.
[3] Hu YH, Zhang CY, Xia RH, et al.Prognostic factors of carcinoma ex pleomorphic adenoma of the salivary glands, with emphasis on the widely invasive carcinoma: a clinicopathologic analysis of 361 cases in a Chinese population[J]. Oral Surg Oral Med Oral Pathol Oral Radiol, 2016, 122(5): 598-608.
[4] 夏亮, 汪洋, 胡宇华, 等. 唾液腺恶性多形性腺瘤HER2基因扩增与临床病理特征及预后的关系[J]. 中国口腔颌面外科杂志, 2018, 16(1): 12-19.
[5] Villegas SL, Darb-Esfahani S, von Minckwitz G, et al. Expression of Cyclin D1 protein in residual tumor after neoadjuvant chemotherapy for breast cancer[J]. Breast Cancer Res Treat, 2018, 168(1): 179-187.
[6] Khabaz MN, Abdelrahman AS, Butt NS, et al.Cyclin D1 is significantly associated with stage of tumor and predicts poor survival in endometrial carcinoma patients[J]. Ann Diagn Pathol, 2017, 30(1): 47-51.
[7] Jour G, West K, Ghali V, et al.Differential expression of p16(INK4A) and cyclin D1 in benign and malignant salivary gland tumors: a study of 44 Cases[J]. Head Neck Pathol, 2013, 7(3): 224-231.
[8] 尚文军, 王洋, 刘丽敏, 等. ERK通路抑制剂苯乙酸钠对转基因小鼠唾液腺肿瘤的作用[J]. 中国口腔颌面外科杂志, 2013, 11(5): 359-364.
[9] Wang Y, Shang W, Lei X, et al.Opposing functions of PLAG1 in pleomorphic adenoma: a microarray analysis of PLAG1 transgenic mice[J]. Biotechnol Lett, 2013, 35(9): 1377-1385.
[10] Zheng Q, Lin Z, Xu J, et al.Long noncoding RNA MEG3 suppresses liver cancer cells growth through inhibiting beta-catenin by activating PKM2 and inactivating PTEN[J]. Cell Death Dis, 2018, 9(3): 253-271.
[11] Mitra R, Chen X, Greenawalt EJ, et al.Decoding critical long non-coding RNA in ovarian cancer epithelial-to-mesenchymal transition[J]. Nat Commun, 2017, 8(1): 1604-1615.
[12] Gupta RA, Shah N, Wang KC, et al.Long non-coding RNA HOTAIR reprograms chromatin state to promote cancer metastasis[J]. Nature, 2010, 464(7291): 1071-1076.
[13] 徐万林, 刘丽敏, 卢浩, 等. PLAG1转基因小鼠中差异表达长链非编码RNA的筛选分析[J]. 中国口腔颌面外科杂志, 2019, 17(1): 20-25.
[14] Ponting CP, Oliver PL, Reik W.Evolution and functions of long noncoding RNAs[J]. Cell, 2009, 136(4): 629-641.
[15] Chen Z, Lin S, Li JL, et al.CRTC1-MAML2 fusion-induced lncRNA LINC00473 expression maintains the growth and survival of human mucoepidermoid carcinoma cells[J]. Oncogene, 2018, 37(14): 1885-1895.
[16] 何巍, 何伟, 曹选平, 等. 细胞周期素D1在唾液腺腺样囊性癌中的表达及意义[J].华西口腔医学杂志, 2005, 23(3): 191-193.
[17] Liu T, Zhu E, Wang L, et al.Abnormal expression of Rb pathway-related proteins in salivary gland acinic cell carcinoma[J]. Hum Pathol, 2005, 36(9): 962-970.
[18] Liu W, Zhu E, Wang R, et al.Cyclin D1 gene polymorphism, A870G, is associated with an increased risk of salivary gland tumors in the Chinese population[J]. Cancer Epidemiol, 2011, 35(4): e12-17.
[19] Zhou JJ, Cheng D, He XY, et al.Knockdown of Hotair suppresses proliferation and cell cycle progression in hepatocellular carcinoma cell by downregulating CCND1 expression[J]. Mol Med Rep, 2017, 16(4): 4980-4986.
[20] Yu T, Shan TD, Li JY, et al.Knockdown of linc-UFC1 suppresses proliferation and induces apoptosis of colorectal cancer[J]. Cell Death Dis, 2016, 7(5): e2228-2236.
[21] Fan S, Yang Z, Ke Z, et al.Downregulation of the long non-coding RNA TUG1 is associated with cell proliferation, migration, and invasion in breast cancer[J]. Biomed Pharmacother, 2017, 95: 1636-1643.