Study on the differentially expressed long non-coding RNAs in PLAG1 transgenic mice by gene micro-array

doi:10.19438/j.cjoms.2019.01.004

Abstract

Abstract: PURPOSE: To screen for the differentially expressed long non-coding RNAs (LncRNAs) in pleomorphic adenoma of PLAG1 transgenic mice. METHODS: Three pairs of pleomorphic adenoma, non-tumor gland tissues from PLAG1 transgenic mice and normal gland tissues of submandibular gland from wide-type mice were collected and then the total RNA was extracted. cDNA probe was synthesized by reverse transcription and hybridized as the cDNA microarray for scanning fluorescent signals and differentially expressed LncRNAs. The expressions of 5 randomly selected LncRNAs were validated in another 6 pairs of tissues via real-time PCR. The expression differences were compared and analyzed using SPSS13.0 software package. RESULTS: Microarray results demonstrated that a total of 5627 LncRNAs (2871 upregulated and 2756 downregulated) were differentially expressed in the tumors of PLAG1 transgenic mice. Real-time PCR data showed that the expression of 4 selected LncRNAs (AK146794, ENSMUST00000119440, ENSMUST00000140495 and Gm12873) were consistent with the microarray results. CONCLUSIONS: Analysis of differentially expressed LncRNAs in PLAG1 transgenic mice can provide evidence for pathogenesis of pleomorphic adenoma.

Key words: Microarray, Pleomorphic adenoma, PLAG1 transgenic mice, Long non-coding RNA

CLC Number:

R739.8

XU Wan-lin, LIU Li-min, LU Hao, YANG Wen-jun, SHEN Shu-kun. Study on the differentially expressed long non-coding RNAs in PLAG1 transgenic mice by gene micro-array[J]. China Journal of Oral and Maxillofacial Surgery, 2019, 17(1): 20-25.

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