Expression and significance of p21 Waf1/Cip1 during the disappearance of MEE/MES in lateral palatine process

Abstract

Abstract: PURPOSE: To investigate the expression of p21^Waf1/Cip1（p21） related protein Notch1, Jag1, Jag2 and p21 in lateral palatine process of normal embryonic mouse and embryonic mouse with cleft palate, especially in MEE/MES, and explore the significance of p21 during the disappearance of MEE/MES. METHODS: Embryonic mouse with cleft palate was induced by all-trans retinoic acid (atRA), the control group was given the equivalent volume of corn oil. The embryonic palates on the 13.5 gestation day (GD13.5), GD14.5, GD15.5 and GD16.5 were obtained. The specimens were observed under acridine orange staining. The expression of p21, Notch1, Jag1 and Jag2 was detected by immunehistochemistry. RESULTS: The expression of p21, Notch1, Jag1 and Jag2 in the experimental group was negative in MEE/MES cells on each gestation day, while it was positive in the control group on GD14.5 and GD15.5. On GD15.5, palate was fused with the disappearance of MES in the control group, whereas MEE / MES did not completely subside in the experimental group. On GD16.5, palate was fused completely in the control group while palate was not fuse in the experimental group, leading to cleft palate. The expression of Jag1, Jag2 and Notch1 was still positive in all epithelium of lateral palatine process. However, p21 only appeared in the nasal side. Palate fused completely in control group while palate was not fused in the experimental group, leading to cleft palate. CONCLUSIONS: This study showed that the expression of p21, Notch1, Jag1 and Jag2 was differential in specific time and space in the epithelium of embryonic mouse lateral palatine process, and p21 was involved in apoptosis and differentiation in lateral palatine process epithelia, especially in the process of ΔNp63 regulating the disappearance of MEE/MES.

Key words: p21 Waf1/Cip1, ΔNp63, Notch, Cleft palate, Medical edge epithelial, Medical epithelial seam

CLC Number:

R782.2

LI Hong-yu, ZHANG Ya-dong, DONG Shi-yi, WANG Wei-cai, WANG Jian-ning, WANG Miao, HUANG Hong-zhang. Expression and significance of p21^Waf1/Cip1 during the disappearance of MEE/MES in lateral palatine process[J]. China Journal of Oral and Maxillofacial Surgery, 2015, 13(2): 110-116.

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Expression and significance of p21^Waf1/Cip1 during the disappearance of MEE/MES in lateral palatine process

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