Effect of RNA interference of TEAD gene on the proliferation and invasion of Tca8113 cell line

China Journal of Oral and Maxillofacial Surgery ›› 2014, Vol. 12 ›› Issue (2): 112-115.

• Basic Research Articles • Previous Articles Next Articles

Effect of RNA interference of TEAD gene on the proliferation and invasion of Tca8113 cell line

XU Xian-yin, LIU Yang, YU Jun

Department of Stomatology, Wuxi People’s Hospital. Wuxi 214023, Jiangsu Province, China

Received:2013-07-15 Revised:2013-09-12 Online:2014-03-10 Published:2014-11-06
Supported by:
Science and Technology Development Fund of Nanjing Medical University(2013NJNU158)

Abstract

Abstract: PURPOSE: To investigate the effect of TEAD gene on biological behavior of tongue squamous cell carcinoma by endogenous TEAD gene knockdown using RNA interference technique in the tongue carcinoma cell line Tca8113. METHODS: Specific iRNA of TEAD gene was established and transfected into the cell line Tca8113. The TEAD gene expression in Tca8113 was detected after transfection using RT-PCR. Cell proliferation and migration were observed by CCK8 and Transwell methods, respectively. The experimental data was statistically analyzed by One-Way ANOVA with SPSS13.0 software package. RESULTS: After siRNA interference, Tca8113 cell line showed significantly decreased expression of TEAD gene (P<0.05), lower rate of proliferation (P<0.05), and weakened invasion (P<0.05) in vitro. CONCLUSIONS: The inhibition of TEAD gene expression by RNAi can suppress the growth, proliferation and migration of Tca8113 cell line, indicating a pivot role of TEAD gene in the pathogenesis of tongue squamous cell carcinoma.

Key words: Tongue carcinoma, Biological behavior, TEAD gene, RNA interference

CLC Number:

R739.8

XU Xian-yin, LIU Yang, YU Jun. Effect of RNA interference of TEAD gene on the proliferation and invasion of Tca8113 cell line[J]. China Journal of Oral and Maxillofacial Surgery, 2014, 12(2): 112-115.

References

[1] Davidson BJ, Root WA, Trock BJ. Age and survival from squamous cell carcinoma of the oral tongue [J]. Head Neck, 2001, 23(4): 273-279.
[2] Allocati N, Di Ilio C, De Laurenzi V. p63/p73 in the control of cell cycle and cell death [J]. Exp Cell Res, 2012, 318(11): 1285-1290.
[3] Zheng HX, Cai YD, Wang YD, et al. Fas signaling promotes motility and metastasis through epithelial-mesenchymal transition in gastrointestinal cancer [J]. Oncogene, 2013, 32(9): 1183-1192.
[4] Tremblay AM, Camargo FD. Hippo signaling in mammalian stem cells [J]. Semin Cell Dev Biol, 2012, 23(7): 818-826.
[5] Karpowicz P, Perez J, Perrimon N. The Hippo tumor suppressor pathway regulates intestinal stem cell regeneration [J]. Development, 2010, 137(24): 4135-4145.
[6] Cherrett C, Furutani-Seiki M, Bagby S. The Hippo pathway: key interaction and catalytic domains in organ growth control, stem cell self-renewal and tissue regeneration [J]. Essays Biochem, 2012, 53: 111-127.
[7] Pan D. The Hippo signaling pathway in development and cancer [J]. Dev Cell, 2010, 19(4): 491-505.
[8] Zhao B, Li L, Lei Q, et al. The Hippo-YAP pathway in organ size control and tumorigenesis: an updated version [J]. Genes Dev, 2010, 24(9): 862-874.
[9] Wisotzkey RG, Konikoff CE, Newfeld SJ. Hippo pathway phylogenetics predicts monoubiquitylation of Salvador and Merlin/nf2 [J]. PLoS One, 2012, 7(12): e51599.
[10] Zhao B, Ye X, Yu J, et al. TEAD mediates YAP-dependent gene induction and growth control [J]. Genes Dev, 2008, 22(14): 1962-1971.
[11] Landin Malt A, Cagliero J, Legent K, et al. Alteration of TEAD1 expression levels confers apoptotic resistance through the transcriptional up-regulation of Livin [J]. PLoS One, 2012, 7(9): e45498.
[12] Liu-Chittenden Y, Huang B, Shim JS, et al. Genetic and pharmacological disruption of the TEAD-YAP complex suppresses the oncogenic activity of YAP [J]. Genes Dev,2012, 26(12): 1300-1305.

Effect of RNA interference of TEAD gene on the proliferation and invasion of Tca8113 cell line

Knowledge

Abstract

Cite this article

share this article

References

Related Articles 3

Recommended Articles

Metrics

Comments

[1]	KONG Xian-chen, JIANG Xiao-dong, LU Xin, YU Wen-yi, XUE Jiao, ZHANG Peng, ZHAO Lu, YUAN Rong-tao. Expression and significance of ribosomal protein L29 in tongue squamous cell carcinoma [J]. China Journal of Oral and Maxillofacial Surgery, 2021, 19(2): 110-115.
[2]	ZHENG Chen, SHI Chao-ji, ZHENG Bo, JIN Xiao-ming, JIANG Yin-hua.. Expression of Smad7 protein in oral squamous cell carcinoma and the impact on cell proliferation and migration of oral squamous cell carcinoma [J]. China Journal of Oral and Maxillofacial Surgery, 2017, 15(3): 220-226.
[3]	ZHOU Rong, SHI Chao-ji, HAN Yong, TAO Wen-jie, LI Jiang, SUN Shu-yang, ZHANG Lei, ZHANG Zhi-yuan. Yes-associated protein overexpression in oral squamous cell carcinoma and association with tumor cell migration and invasion [J]. China Journal of Oral and Maxillofacial Surgery, 2016, 14(3): 203-208.