Effect of static pressure on expression of COL2A1, SOX9, COL1A1 and ALP in temporomandibular condylar chondrocytes in rabbit

Abstract

Abstract: PURPOSE: To study the cellular events of condylar chondrocytes during the alteration of pressure microenvironment. METHODS: Condylar chondrocytes were aseptically dissected and cultured from the temporomandibular joint (TMJ) of 4-week-old New Zealand white rabbit in vitro. The chondrocytes of P2 were identified by morphological analysis, immunocytochemical staining of COL2A1 and SOX9. The monolayer of condylar chondrocytes was subjected to static pressure of 100kPa for different times (0 h, 1 h, 2 h, 3 h and 4 h). The viability of chondrocytes was assessed by CCK-8 staining. Expression of COL2A1, SOX9, COL1A1 and ALP was examined by Western blot. One-way ANOVA was performed using SPSS19.0 software package. RESULTS: The condylar chondrocytes of rabbits were polygon in shape and displayed cobble-stone morphology. Immunocytochemical staining of COL2A1 and SOX9 was positive in the slides of cells. Compared with 0 h, the viability of chondrocytes significantly decreased after being compressed for 1 h (P=0.04), whereas it returned to normal after 2 to 4 h. Western blot results showed that exposure of chondrocytes to 100 kPa for 3 h and 4 h resulted in significant up-regulation of COL2A1, SOX9, COL1A1 and ALP. Moreover, the expression of ALP at 4 h reduced compared with at 3 h. CONCLUSIONS: The condylar chondrocytes are good at adapting to the alteration of pressure microenvironment. It is sure that a suitable static pressure loading can accelerate chondrogenesis and osteogenesis. And this may influence the growth remodeling of TMJ and the pathological condition of temporomandibular joint disorder (TMD).

Key words: Condyle, Chondrocytes, Static pressure, COL2A1, SOX9, COL1A1, ALP

CLC Number:

R782.2

HUANG Lin-jian, LI Hui, XIE Qian-yang, ZHANG Min, CHENG Yong-jin, YANG Chi, CAI Xie-yi. Effect of static pressure on expression of COL2A1, SOX9, COL1A1 and ALP in temporomandibular condylar chondrocytes in rabbit[J]. China Journal of Oral and Maxillofacial Surgery, 2014, 12(4): 295-300.

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