Expression and significance of DLK2 gene during cartilage development

Abstract

Abstract: PURPOSE: To study the expression and significance of DLK2 during embryonic cartilage development. METHODS: The expression of DLK2 was detected during the differentiation of ATDC5 cells. ATDC5-DLK2 cells which stably overexpressed DLK2 was constructed by lentivirus transfection, and the effect of DLK2 on the proliferation and differentiation of ATDC5 cells was studied. The temporal and spatial expression of DLK2 in cartilage of C57BL/6 mouse at embryos(E11.5,E16.5,E18.5 days) and postnatal time (P7 and P14 day) was examined by immunohistochemistry. The data was analyzed by paired t test using SPSS16.0 software package. RESULTS: The mRNA and protein levels of DLK2 decreased during the differentiation of ATDC5 cells; ATDC5-DLK2 cell line which stably ovexpressed DLK2 was established and identified, and DLK2 overexpression promoted the proliferation of ATDC5 cells (P<0.05),but significantly inhibited the expression of chondrogenic marker genes (col2a1, acan, col10a1)(P<0.05). DLK2 showed a weak expression in cartilage of early embryo (E11.5 days), while gradually increased during the development of cartilage. DLK2 was expressed in both immature and mature chondrocytes, but the expression of DLK2 in articular cartilage surface decreased (P<0.05), while almost unchanged in the deep of articular cartilage (P>0.05). CONCLUSIONS: DLK2 may be an important factor involved in cartilage development.

Key words: DLK2, ATDC5 cells, Cartilage development

CLC Number:

R786.2

XU Wei-feng, SHEN Pei, FAN Bao-ting, JIAO Zi-xian, LI Hui-ping, ZHANG Shan-yong. Expression and significance of DLK2 gene during cartilage development[J]. China Journal of Oral and Maxillofacial Surgery, 2016, 14(4): 302-307.

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