Effect of RANKL on chondrocyte differentiation of temporomandibular joint

doi:10.19438/j.cjoms.2017.02.001

China Journal of Oral and Maxillofacial Surgery ›› 2017, Vol. 15 ›› Issue (2): 97-102.doi: 10.19438/j.cjoms.2017.02.001

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Effect of RANKL on chondrocyte differentiation of temporomandibular joint

FAN Bao-ting^{1, 2}, XU Wei-feng¹, SHEN Pei¹, LI Hui-ping¹, ZHAO Hua-qiang², ZHANG Shan-yong¹

1.Department of Oral Surgery, Shanghai Ninth People's Hospital, College of Stomatology, Shanghai Jiao Tong University School of Medicine; Shanghai Key Laboratory of Stomatology. Shanghai 200011;
2.Department of Oral and Maxillofacial Surgery, School of Stomatology, Shandong University. Jinan 250012, Shandong Province, China

Received:2016-09-20 Online:2017-03-20 Published:2017-04-13
Contact: 国家自然科学基金（81371168,81671010）; 上海交通大学医工交叉项目（YG2013MS63）; 上海市教育委员会高峰-高原学科-上海交通大学医学院“研究型医师”项目（20152226）; 上海交通大学医学院转化医学创新基金（15ZH2007）; 上海市科学技术委员会生物医药支撑项目（16441908800）; 上海市卫生和计划生育委员会重点项目（201640001）; 科技部国家重点研发计划项目（2016YFC1100600）

Abstract

Abstract: PURPOSE : This study was aimed to identify a novel therapeutic way for joint cartilage degeneration. METHODS : By using ATDC5 chondrogenic cells and bovine cartilages, we studied the role of RANKL in cartilage degeneration in vitro. The protein expression of MMP13, RANK, RANKL and ADAMTS5 was detected using Western blot. The mRNA expression of Col2a1, Col10a, MMP13, RANK and ADAMTS5 was detected using RT-qPCR. Degeneration of cartilage was analyzed by safranin O and alician blue staining and Mankin score. The data were analyzed by paired t test using SPSS17.0 software package. RESULTS : The results of real time PCR confirmed that both RANK and RANKL were dynamically expressed in ATDC5 cells during all differentiation phases (P<0.05). RANK protein expression was significantly enhanced by RANKL treatment for 48 h in comparison to that for 0h. The mRNA and protein level of ADAMTS5 was significantly increased as stimulated by RANKL compared with the control over time (P<0.05). In addition, vacuolation and degeneration were observed in bovine cartilages with RANKL stimulation (P<0.05). CONCLUSION S: RANKL contributes to cartilage degeneration in condylar resorption and inhibition of this pathway may be a useful strategy for condylar resorption treatment.

CLC Number:

R786.2

FAN Bao-ting, XU Wei-feng, SHEN Pei, LI Hui-ping, ZHAO Hua-qiang, ZHANG Shan-yong. Effect of RANKL on chondrocyte differentiation of temporomandibular joint[J]. China Journal of Oral and Maxillofacial Surgery, 2017, 15(2): 97-102.

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Effect of RANKL on chondrocyte differentiation of temporomandibular joint

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