Establishment of infantile hemangioma on nude mice with xenograft of human hemangioma tissue

Abstract

Abstract: PURPOSE : To find a way to establish a human hemangioma model on nude mice. METHODS : Sixteen BALB/c nude mice were randomly divided into 2 groups. In one group, the mice were administered intramuscularly with 0.01 mg estradiol every week. Additionally, and another group with normal diet was as control. The specimen of human maxillofacial hemangioma was inoculated subcutaneously into 16 nude mice. The changes of hemangioma tissues were observed and harvested on the 90th day after graft, and then subjected to histopathological examination and immunohistochemical staining for CD31, CD34, Ki67. Statistical analysis was performed using SPSS 13.0 software package. RESULTS : It was found that the volume of hemangioma tissues in the experimental group increased more greatly than the control group, and the growth characteristics were similar to human hemangioma. Statistical differences were found between hemangioma tissues and surrounding normal muscle tissues in CD31, CD34, Ki67 staining. CONCLUSIONS : It is possible to establish an experimental model of human infantile hemangioma by implanting human hemangioma tissue into nude mice, which could lay a foundation for further study of human hemangioma.

Key words: Infantile hemangioma, Animal model, Immunohistochemistry

CLC Number:

R739.8

PENG Hao, ZHOU Guo-yu, ZHAO Jian-xin, SHEN Ling-yue, MA Chuan.. Establishment of infantile hemangioma on nude mice with xenograft of human hemangioma tissue[J]. China Journal of Oral and Maxillofacial Surgery, 2015, 13(6): 486-490.

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