The mechanism of lipopolysaccharide upregulating trigeminal ganglionic sodium channel 1.7 expression in rats

doi:10.19438/j.cjoms.2023.04.005

Abstract

Abstract: PURPOSE: To investigate whether and how lipopolysaccharide(LPS) upregulates the expression of sodium channel 1.7 (Nav1.7) in trigeminal ganglion (TG), and then participate in temporomandibular joint (TMJ) inflammatory pain in rats. METHODS: Through microinjection of LPS into the TG in adult male Sprague-Dawley (SD) rats, rats' head withdrawal threshold was observed and the expression of Nav1.7 in TG was detected. After culturing rats TG explants in vitro, the rats TG explants were treated with LPS with or without inhibitors, including fluorocitrate(FC) for satellite glial cell (SGC) activation and pyrrolidinedithiocarbamate (PDTC) for NF-κB, and then the expression changes of Nav1.7, p-p65 and GFAP in TG was detected using real-time PCR and Western blot assays. SPSS 22.0 software package was used for data analysis. RESULTS: Nav1.7 expression was significantly upregulated in the TG and the head withdrawal threshold was reduced after microinjection of LPS into the TG for 24 h. LPS-induced upregulation of Nav1.7 expression in the TG was blocked by SGC activation inhibitor FC. LPS-induced upregulation of p-p65, GFAP and Nav1.7 expressions in the TG were blocked by NF-κB inhibitor PDTC. CONCLUSIONS: LPS upregulates the expression of Nav1.7 through NF-κB signaling pathway (p-p65) activating satellite glial cell (SGC) of trigeminal ganglion, and then participates in TMJ inflammatory pain in rats.

Key words: Lipopolysaccharide, Trigeminal ganglion, Satellite glial cells, Sodium channel 1.7, Temporomandibular inflammatory pain

CLC Number:

R782.6

YAN Ting-ting, ZHANG Jing, ZHUANG Li-kun, WANG Li-li, ZHANG Peng, YUAN Rong-tao. The mechanism of lipopolysaccharide upregulating trigeminal ganglionic sodium channel 1.7 expression in rats[J]. China Journal of Oral and Maxillofacial Surgery, 2023, 21(4): 345-351.

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