The role of MAP7D3 in head and neck squamous cell carcinoma

doi:10.19438/j.cjoms.2023.04.006

Abstract

Abstract: PURPOSE: The aim of this study is to figure out MAP7D3 expression level, diagnostic and prognostic value in head and neck squamous cell carcinoma (HNSCC) by bioinformatics technology and to initially explore the effect of the expression change of MAP7D3 on the biological behavior of HNSCC cell lines. METHODS: Expression analysis, clinicopathological characteristics correlation analysis, survival analysis, Cox regression analysis, ROC curve analysis and GSEA analysis were performed in HNSCC by bioinformatics.The expression of MAP7D3 were detected by qRT-PCR and immunohistochemistry staining in HNSCC cell lines(WSU-HN6, CAL27) and HNSCC tissues. Downregulation of MAP7D3 by siRNA was identified by qRT-PCR. CCK8 assay, flow apoptosis assay, wound healing assay and transwell assay ware performed to detect the effect of knocking down MAP7D3 on the biological behaviors of HNSCC cell lines WSU-HN6 and CAL27. RESULTS: MAP7D3 was highly expressed in HNSCC at both RNA and protein level by bioinformatic analysis, qRT-PCR and immunohistochemical staining. Clinical correlation analysis revealed that the expression level of MAP7D3 was correlated with pathological grade. Overall survival, disease specific survival and progress free interval were shorter in patients with high MAP7D3 expression compared with patients with low MAP7D3 expression. Univariate and multivariate Cox regression analysis showed that MAP7D3 expression could be an independent risk factor for prognosis of HNSCC patients. ROC curves showed the diagnostic value of MAP7D3 in differentiating HNSCC tissue from normal tissue. GSEA enrichment analysis showed that MAP7D3 high expression group was enriched in various signaling pathways such as focal adhesion and ECM-receptor interactions. CCK-8 assay, flow apoptosis assay, wound healing assay and transwell assay showed that knockdown of MAP7D3 can inhibit the proliferation, migration, invasion and increase apoptosis of HNSCC cell lines WSU-HN6 and CAL27. CONCLUSIONS: MAP7D3 is highly expressed in HNSCC and can be used as a potential diagnostic and prognostic biomarker for HNSCC. Downregulation of MAP7D3 can inhibit the proliferation, migration and invasion of HNSCC cell and increase cell apoptosis.

Key words: Head and neck squamous cell carcinoma, Bioinformatics, MAP7D3, Tumor biomarkers

CLC Number:

R739.8

LIU Yu, CHEN Yu-ling, LIN Yun-tao, YANG Hong-yu. The role of MAP7D3 in head and neck squamous cell carcinoma[J]. China Journal of Oral and Maxillofacial Surgery, 2023, 21(4): 352-359.

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