The effect of dietary nitrate on rat dorsal random flap and its mechanism

doi:10.19438/j.cjoms.2021.02.002

Abstract

Abstract: PURPOSE: To investigate the effect of oral nitrate on the survival of rat dorsal random flap, and to explore its potential mechanism by taking nitrate-nitrite-NO pathway as the breakthrough point. METHODS: Twenty-four healthy adult male Wistar rats were randomly divided into 3 groups (n=8). Modified McFarlane rat dorsal random skin flap was used to make the model. The experimental group was given 0.5 mmol/L sodium nitrate 7 days before operation and daily after operation, while the control group was given the same amount of sodium chloride daily, and the blank group was given distilled water daily. The survival area of the flap was observed on the 7th postoperative day. On the 7th day after operation, the contents of serum nitrate and nitrite in rats were detected, and the levels of TNF-α and IL-6 in serum were further detected. Meanwhile, histological changes of skin flap were observed and the levels of malondialdehyde(MDA) and superoxide dismutase(SOD) were analyzed in flap tissue samples. The data were analyzed with SPSS software package. RESULTS: The survival area of the flap in the experimental group was significantly higher than that in the control group on the 7th day after operation, and the serum nitrate and nitrite contents were significantly higher than those in the control group. At the same time, H-E staining found that nitrate reduced the histological damage of the flap. Through observation of the expression of serum TNF-α and IL-6, it was found that nitrate reduced inflammatory reaction. In addition, the oxidative stress marker MDA was reduced, while the activity of SOD was restored after nitrate treatment. CONCLUTIONS: Oral nitrate can improve the survival area of random flap in rats. The mechanism is associated by promoting angiogenesis and reducing circulating inflammatory factors through nitrate-nitrite - NO pathway.

Key words: Nitrate, Skin flap, Nitric oxide, Inflammation, Oxidative stress

CLC Number:

R782.2

PANG Bao-xing, BU Ling-xue, JIA Mu-yun, YUAN Rong-tao, SHANG Wei. The effect of dietary nitrate on rat dorsal random flap and its mechanism[J]. China Journal of Oral and Maxillofacial Surgery, 2021, 19(2): 105-109.

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