Decrease of pain threshold of trigeminal nerve induced by reactivation of latent trigeminal ganglia infection of herpes simplex virus type 1 in SD rats

doi:10.19438/j.cjoms.2020.03.005

Abstract

Abstract: PURPOSE: To establish an animal model of latent infection and reactivation of trigeminal ganglia caused by herpes simplex virus type 1 (HSV-1) in SD rats. The effect of HSV-1 infection and reactivation of ganglion on pain threshold of trigeminal nerve was observed by pain threshold test. METHODS: SD rats were randomly divided into the experimental group and the control group. Rats in the experimental group were treated by corneal scratch with HSV-1 to establish latent infection model of ganglion, while rats in the control group were treated by corneal scratch with normal saline. After 8 weeks, the rats in the experimental group were divided into ultraviolet irradiation group and non ultraviolet irradiation group, ultraviolet irradiation was used to induce reactivation of HSV-1. The mechanical pain threshold of each group of rats was measured by von Frey filament pain threshold detector. The expression levels of HSV-gD, LAT and ICP27 were detected by RT-PCR to verify the success of latent infection and reactivation of HSV-1 in ganglion. RESULTS: Latency and reactivation of HSV-1 in ganglion were successful fulfilled in rats; pain like stimulation reaction occurred in rats of the experimental group and pain threshold decreased both in latency and reactivation,especially in reactivation. CONCLUSIONS: Reactivation of latent infection of HSV-1 in trigeminal ganglia can induce the decrease of trigeminal neuralgia threshold, which suggests that HSV-1 may be the cause of trigeminal neuralgia.

Key words: HSV-1, Trigeminal neuralgia, Pain threshold, Etiology

CLC Number:

R745.1.1

LIU Run-ze, YANG Yan-ling, XUE Jin-lang, FANG Zhuo-ran, WANG Yun, DENG Chao, CHEN Chuan-jun. Decrease of pain threshold of trigeminal nerve induced by reactivation of latent trigeminal ganglia infection of herpes simplex virus type 1 in SD rats[J]. China Journal of Oral and Maxillofacial Surgery, 2020, 18(3): 214-218.

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