China Journal of Oral and Maxillofacial Surgery ›› 2017, Vol. 15 ›› Issue (5): 413-415.doi: 10.19438/j.cjoms.2017.05.007

• Original Articles • Previous Articles     Next Articles

Involvement of Maspin methylation in the proliferation of oral squamous cell carcinoma

LUO Jun, WU Wei-li, YING Yu-kang, XU Xu-hui, ZHU Hai-qian.   

  1. Department of Stomatology, Taizhou Central Hospital. Taizhou 318000, Zhejiang Province, China
  • Received:2016-11-28 Online:2017-08-30 Published:2017-10-27

Abstract: PURPOSE: Explore the involvement of Maspin methylation in the proliferation of oral squamous cell carcinoma. METHODS: HIOEC-B(a)P cell line was divided into 4 groups: negative control, ADC+TSA with low (0.1 μmol/L+0.05 μmol/L), middle (1 μmol/L+0.5 μmol/L) and high (10 μmol/L+5 μmol/L) dose. Oral epithelial cells were taken as control. Methylation degree was detected by real-time PCR. The proliferation and apoptosis were detected by MTT and DAPI staining. The data were analyzed using SPSS 20.0 software package. RESULTS: Maspin methylation was enhanced in tumor cells when compared with control group (P<0.01). Maspin methylation was decreased in middle and high dose group when compared with negative group (P<0.05, P<0.01). The inhibition ratio of cell multiplication was enhanced when compared with negative group (P<0.05, P<0.01). The apoptosis content was enhanced after incubation with ADC+TSA with a dose dependent manner (P<0.05). CONCLUSIONS: Methylation of Maspin gene may be involved in the proliferation of oral cancer cells.

Key words: Maspin gene, Methylation, ADC, TSA

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